Development of a protoplast isolation system for functional gene expression and characterization using petals of Camellia oleifera (Record no. 54501)
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| 000 -LEADER | |
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| fixed length control field | 02563nam a2200301Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625162448.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-20406 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Development of a protoplast isolation system for functional gene expression and characterization using petals of Camellia oleifera |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Plant Physiology and BioChemistry, 201, p.107885, 2023 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Protoplasts preparation and purification have been frequently used in plant genetics and breeding studies, whereas application of protoplasts in woody plants is still in its infancy. Although transient gene expression using purified protoplasts is well-documented and widely used in model plants and agriculture crops, no instance of either stable transformation or transient gene expression in the woody plant Camellia Oleifera has as of yet been reported. Here, we developed a protoplast preparation and purification method using C. oleifera petals by optimizing osmotic condition with D-mannitol and polysaccharide-degrading enzyme concentrations for petal cell wall digestion, to reach a high efficiency of protoplast productivity and viability. The achieved protoplasts yield was approximately 1.42 × 107 cells per gram of petal material and the viability of protoplasts was up to 89 percent. In addition, we explored influencing factors of protoplast transformation, including concentrations of PEG4000 and plasmid DNA. The transformation efficiency of 81 percent could be reached under the optimized condition. This protoplast isolation and transient expression system were deployed to further identify the functional regulation of C. oleifera related genes and the subcellular distribution of their encoded products. In summary, the protoplast isolation and transient expression system we established using oil-tea tree petals is an efficient, versatile and time-saving system, being suitable for gene function characterization and molecular mechanism analysis. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CAMELLIA OLEIFERA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | MOLECULAR MECHANISM |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PROTOPLAST PREPARATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PROTOPLAST TRANSIENT EXPRESSION SYSTEM |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PETALS |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Lin, Z. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Huang, L. J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Yu, P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Chen, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Du, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Qin, G. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Yuan, D. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1PN7OL5cx8_kuJNoSWZOhT73NQT_vfUzZ/view?usp=drivesdk">https://drive.google.com/file/d/1PN7OL5cx8_kuJNoSWZOhT73NQT_vfUzZ/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-20406 | 25.06.2025 | 25.06.2025 | Documentos solicitados |