MARC details
| 000 -LEADER |
|---|
| fixed length control field |
02274nam a2200277Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20250625162449.0 |
| 040 ## - CATALOGING SOURCE |
|---|
| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
|---|
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-20477 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Overexpression and characterization of a novel plant carotenoid cleavage dioxygenase 1 from Morus notabilis |
| 490 0# - SERIES STATEMENT |
|---|
| Volume/sequential designation |
Chemistry & BioDiversity, 19(2), p.e202100735, 2022 |
| 520 3# - SUMMARY, ETC. |
|---|
| Summary, etc. |
Synthesis of ?-ionone in microbial cell factories is limited by the efficiency of carotenoid cleavage dioxygenases (CCDs). To obtain genes responsible for specific cleavage of carotenoids generating ?-ionone, a novel carotenoid cleavage dioxygenase 1 from Morus notabilis was cloned and overexpressed in Escherichia coli. The MnCCD1 protein was able to cleave a variety of carotenoids at the positions 9, 10 (9?, 10?)to produce ?-ionone, 3-hydroxy-4-oxo-?-ionone, 3-hydroxy-?-ionone, and 3-hydroxy-?-ionone in vitro. MnCCD1 could also cleave lycopene and ?-carotene at the 9, 10 (9?, 10?)bind bond to produce pseudoionone and ?-ionone, respectively, in E. coli accumulating carotenoids. The enzyme activity of MnCCD1 was reached 2.98 U/mL at optimized conditions (temperature 28?°C, IPTG 0.1 mM, induction time 24 h). The biochemical characterization of MnCCD1 revealed the optimal activities were at pH 8.4 and 35?°C. The addition of 10? percent ethanol could increase enzyme activity at above 15? percent. However, an obvious decline was observed on enzyme activity as the concentration of Fe2+ increased (0-1 mM). The Vmax for ?-apo-8?-carotenal was 72.5 U/mg, while the Km was 0.83 mM. The results provide a foundation for developing the application of carotenoid cleavage dioxygenases as biocatalysis and synthetic biology platforms to produce volatile aroma components from carotenoids. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
MORUS NOTABILIS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
CAROTENOID CLEAVAGE DIOXYGENASES |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
CCD1 |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
B-IONONE |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Qi, Z. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Fan, X. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Zhu, C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Chang, D. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Pei, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Zhao, L. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
|---|
| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1ULG5GOTFyrX9NZZ03VyBo5JZk1GahDv8/view?usp=drivesdk">https://drive.google.com/file/d/1ULG5GOTFyrX9NZZ03VyBo5JZk1GahDv8/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
