Improving Toluene Dioxygenase Activity for Ester-Functionalized Substrates through Enzyme Engineering (Record no. 54570)

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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625162449.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-20479
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Title Improving Toluene Dioxygenase Activity for Ester-Functionalized Substrates through Enzyme Engineering
490 0# - SERIES STATEMENT
Volume/sequential designation ChemistrySelect, 7(11), p.e202200753, 2022
520 3# - SUMMARY, ETC.
Summary, etc. Rieske dioxygenases have a history of utility in organic synthesis, owing to their ability to catalyze the asymmetric dihydroxylation of aromatics to produce chiral diene-diol metabolites. However, their utility as green-chemical tools has been limited by steric and electronic constraints on their substrate scopes and their activity. Herein we report the rational engineering of a widely used Rieske dioxygenase, toluene dioxygenase (TDO), to improve the activity of this enzyme system for the dihydroxylation of a synthetically valuable substrate class for which the wild-type enzyme possesses low activity, the ester-functionalized aromatics. Through active site targeted mutagenesis and application of a recently reported high throughput screening platform, engineered TDO variants with significantly increased activity in the dihydroxylation of these valuable substrates were identified and characterized, revealing key active site residues that modulate the enzyme's activity and selectivity.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Osifalujo, E. A.
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Personal name Preston-Herrera, C.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Betts, P. C.
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Personal name Satterwhite, L. R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Froese, J. T.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1ldz7jIq9AJ2THmf1AquvjLOcqDyHnpEz/view?usp=drivesdk">https://drive.google.com/file/d/1ldz7jIq9AJ2THmf1AquvjLOcqDyHnpEz/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-20479 25.06.2025 25.06.2025 Documentos solicitados