miR171-targeted SCARECROW-LIKE genes CsSCL2 and CsSCL3 regulate somatic embryogenesis in citrus (Record no. 54632)

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fixed length control field 02361nam a2200241Ia 4500
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control field MX-MdCICY
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control field 20250625162450.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-20543
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Title miR171-targeted SCARECROW-LIKE genes CsSCL2 and CsSCL3 regulate somatic embryogenesis in citrus
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Volume/sequential designation Plant Physiology, 192, p.2838-2854, 2023
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Summary, etc. Somatic embryogenesis (SE)is a key regeneration pathway in various biotechnology approaches to crop improvement, especially for economically important perennial woody crops like citrus. However, maintenance of SE capability has long been a challenge and becomes a bottleneck in biotechnology-facilitated plant improvement. In the embryogenic callus (EC)of citrus, we identified 2 csi-miR171c-targeted SCARECROW-LIKE genes CsSCL2 and CsSCL3 (CsSCL2/3), which exert positive feedback regulation on csi-miR171c expression. Suppression of CsSCL2 expression by RNA interference (RNAi)enhanced SE in citrus callus. A thioredoxin superfamily protein CsClot was identified as an interactive protein of CsSCL2/3. Overexpression of CsClot disturbed reactive oxygen species (ROS)homeostasis in EC and enhanced SE. Chromatin immunoprecipitation sequencing (ChIP-Seq)and RNA-Seq identified 660 genes directly suppressed by CsSCL2 that were enriched in biological processes including development-related processes, auxin signaling pathway, and cell wall organization. CsSCL2/3 bound to the promoters of regeneration-related genes, such as WUSCHEL-RELATED HOMEOBOX 2 (CsWOX2), CsWOX13, and Lateral Organ Boundaries Domain 40 (LBD40), and repressed their expression. Overall, CsSCL2/3 modulate ROS homeostasis through the interactive protein CsClot and directly suppress the expression of regeneration-related genes, thus regulating SE in citrus. We uncovered a regulatory pathway of miR171c-targeted CsSCL2/3 in SE, which shed light on the mechanism of SE and regeneration capability maintenance in citrus.
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Personal name Feng, M. Q.
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Personal name Jiang, N.
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Personal name Wang, P. B.
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Personal name Liu, Y.
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Personal name Xia, Q. M.
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Personal name Jia, H. H.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Wu, X. M.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1xRUc9fyLO6SnYChNvGCHD1FkSRM6zA0N/view?usp=drivesdk">https://drive.google.com/file/d/1xRUc9fyLO6SnYChNvGCHD1FkSRM6zA0N/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-20543 25.06.2025 25.06.2025 Documentos solicitados