MARC details
| 000 -LEADER |
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| fixed length control field |
02832nam a2200313Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625162451.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-20553 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
The de novo transcriptome assembly of Agave angustifolia Haw, mining for carbohydrates and cellulose synthesis genes profiling, and molecular markers development |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Plant Biotechnology Reports, https://doi.org/10.1007/s11816-023-00861-6, 2023 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Although Agave angustifolia Haw is one of the most cultural and industrial promising species in Mexico, no extensive genomic resources were available. Herein, we explore the transcriptome data set of this species. Total RNA from stem, roots, young and mature leaves was purified and sequenced by NovaSeq6000 system. From the de novo assembly of A. angustifolia, a total of 358,170 transcripts with average length of 1117 bp, N50 of 858 bp, and GC mean content of 43.5 percent were obtained. About 48.7 percent of the transcripts had open reading frame (ORF). Hence, 63.4 percent and 42.7 percent of the ORFs were annotated using Blastp and Blastx against UniProtKB database, respectively, uncovering the presence of at least 29,273 genes in the A. angustifolia transcriptome. The six pairwise comparisons between plant tissues resulted in the identification of 123,181 differentially expressed transcripts (23,978 were upregulated and 79,203 were downregulated). Several carbohydrate metabolism pathways were significantly enriched from the comparisons between leaves and stems. Thus, genes regulated for the fructan, starch and sucrose biosynthesis were discussed. Furthermore, genes involved in cellulose synthesis were explored being the members of the cellulose synthase genes subfamily were highly expressed in young leaf, while the members of the cellulose synthase-like subfamily were preferentially expressed in mature leaf. Moreover, A. angustifolia transcriptome served for the identification of 30,766 SSR and 21,686 VNTR potential markers. Overall, the transcriptomic data generated in this study provide an invaluable resource to understand the biology of A. angustifolia, offering new insight to understand their physiology and adaptation. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
RNA-SEQ |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
GENE EXPRESSION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
AGAVE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
FRUCTAN |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CELLULOSE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
SSRS |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Valadez-Moctezuma, E. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Samah, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Mascorro-Gallardo, J. O. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Reynoso, J. J. L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Marbán-Mendoza, N. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Aranda-Osorio, G. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Brito-Nájera, G. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1ViqDWiqCcuLUbO6_jnHkSMIcaVgcYkaX/view?usp=drivesdk">https://drive.google.com/file/d/1ViqDWiqCcuLUbO6_jnHkSMIcaVgcYkaX/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
