Transcriptome and physiological analysis highlights the hormone, phenylpropanoid, and photosynthesis effects on early somatic embryogenesis in Ginkgo biloba (Record no. 54644)
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| 000 -LEADER | |
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| fixed length control field | 02997nam a2200289Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625162451.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-20557 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Transcriptome and physiological analysis highlights the hormone, phenylpropanoid, and photosynthesis effects on early somatic embryogenesis in Ginkgo biloba |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Industrial Crops and Products, 203, p.117176, 2023 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | The somatic embryogenesis (SEs)system in Ginkgo biloba L. has not been fully established due to the recalcitrance of this species, and it has seriously limited the application of SEs technology, such as elite varieties large-scale production, gene editing, or transformation. Recently, we have induced the cotyledon-shaped somatic embryos (SE)of G. biloba, which was so far observed the closest to mature somatic embryos. Here, we performed the physiological analysis in six cultured stages during SEs formation, including d-NEC (non-embryogenic callus), d-EC (embryogenic callus), d-SE, d-WHC (while hairy callus), L-EC (EC in light), and L-SE (SE in light). The transcriptomic analysis in d-NEC, d-EC, L-EC, and d-WHC was also carried out. We found that the higher (IAA+ZR+iPA)/ABA and the lower IAA/(iPA+ZR)levels were critical in the somatic embryogenesis of G. biloba. The higher levels of soluble sugar, total flavonoid, terpene lactone, PAL, F3'H, and CCR were beneficial to somatic embryogenesis. In addition, the peroxidase (POD)might be related to the cell wall formation of somatic embryos in G. biloba. A total of 36517 unigenes were identified in four groups by transcriptome analysis, and genes differentially expressed (DEGs)were related to hormone homeostasis i.e., SAUR50, Jasmonic acid synthesis i.e., OPR3, phenylpropanoid biosynthesis i.e., CCR1, POD43 and C4H, terpenoid backbone biosynthesis i.e., HMGS, starch and sucrose metabolism, i.e., coniferin ?-glucosidase, and photosynthesis, i.e., D1 protein. Furthermore, the majority of identified up-regulated transcription factors (TFs)such as MYB4, WRKY43, EFR8, and WUS might be responsible for the acquisition of SEs. The light was helpful to the early formation of somatic embryos, and the gene GbWUS1 and GbCLV3 were detected with higher expression in early SE induction of G. biloba. This finding will provide important theoretical and technical support for gene editing, cell totipotency research, and rapid propagation in G. biloba. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | GINKGO BILOBA L |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | SOMATIC EMBRYOGENESIS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | EMBRYOGENIC CALLUS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | TRANSCRIPTOME AND PHYSIOLOGICAL ANALYSIS |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Chen, Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hu, Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wang, R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Feng, K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Di, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Feng, T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Cao, F. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1JVVr-4qCBD8BS5pBWtVdKOYCLhXB3Za0/view?usp=drivesdk">https://drive.google.com/file/d/1JVVr-4qCBD8BS5pBWtVdKOYCLhXB3Za0/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-20557 | 25.06.2025 | 25.06.2025 | Documentos solicitados |