MARC details
| 000 -LEADER |
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| fixed length control field |
02620nam a2200349Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625162451.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-20563 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Isolation and characterization of barley (Hordeum vulgare)extracellular vesicles to assess their role in RNA spray-based crop protection |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
International Journal of Molecular Sciences, 22(13), p.7212, 2021 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
The demonstration that spray-induced gene silencing (SIGS)can confer strong disease resistance, bypassing the laborious and time-consuming transgenic expression of double-stranded (ds)RNA to induce the gene silencing of pathogenic targets, was ground-breaking. However, future field applications will require fundamental mechanistic knowledge of dsRNA uptake, processing, and transfer. There is increasing evidence that extracellular vesicles (EVs)mediate the transfer of transgene-derived small interfering (si)RNAs in host-induced gene silencing (HIGS)applications. In this study, we establish a protocol for barley EV isolation and assess the possibilities for EVs regarding the translocation of sprayed dsRNA from barley (Hordeum vulgare)to its interacting fungal pathogens. We found barley EVs that were 156 nm in size, containing predominantly 21 and 19 nucleotide (nts)siRNAs, starting with a 5'-terminal Adenine. Although a direct comparison of the RNA cargo between HIGS and SIGS EV isolates is improper given their underlying mechanistic differences, we identified sequence-identical siRNAs in both systems. Overall, the number of siRNAs isolated from the EVs of dsRNA-sprayed barley plants with sequence complementarity to the sprayed dsRNA precursor was low. However, whether these few siRNAs are sufficient to induce the SIGS of pathogenic target genes requires further research. Taken together, our results raise the possibility that EVs may not be mandatory for the spray-delivered siRNA uptake and induction of SIGS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
FUSARIUM GRAMINEARUM |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
RNA INTERFERENCE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
RNA SPRAY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
RNAI |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
BARLEY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
DSRNA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
EXTRACELLULAR VESICLES |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
PLANT EV |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
SIRNA |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Schlemmer, T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Barth, P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Weipert, L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Preußer, C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Hardt, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Möbus, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Koch, A. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
|---|
| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1uQgA3jBJXR_0i4tOLdhZ54ZTqJpUVLGx/view?usp=drivesdk">https://drive.google.com/file/d/1uQgA3jBJXR_0i4tOLdhZ54ZTqJpUVLGx/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
