Influence of fermentation reactions on continuous hydrogen photoproduction by microalga Chlamydomonas reinhardtii under sulfur deficiency (Record no. 54689)

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control field 20250625162452.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-20603
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245 10 - TITLE STATEMENT
Title Influence of fermentation reactions on continuous hydrogen photoproduction by microalga Chlamydomonas reinhardtii under sulfur deficiency
490 0# - SERIES STATEMENT
Volume/sequential designation Moscow University Biological Sciences Bulletin, 77(1), p.25-31, 2022
520 3# - SUMMARY, ETC.
Summary, etc. The study investigated the effect of the major fermentation enzyme, pyruvate formate lyase, on hydrogen photoproduction by green microalga Chlamydomonas reinhardtii under mineral deprivation. Cultures of the wild type (CC-125)and mutant pfl1 without pyruvate formate lyase activity were incubated on a medium without sulfur in the sealed photoreactors under constant illumination for 96 h. During the experiment, accumulation of hydrogen in a gas phase of photoreactors and factors affecting hydrogen production, such as photosynthetic activity, respiration, and starch content, in cells were assessed. Both strains showed similar photosynthetic activity and starch content when cultivated in the complete medium. Under sulfur depletion, the cultures of the pfl1 mutant began to evolve hydrogen later than the wild type; however, this process lasted much longer in the mutant. The pfl1 cultures showed higher rates of starch accumulation and breakdown during the initial and late stages of sulfur deprivation, respectively. Moreover, the mutant was characterized by higher photosynthetic activity at the later stage of deprivation. The obtained results indicate close relationships between photosynthetic electron transport, hydrogenase reaction, carbohydrate metabolism and fermentation process, pointing to the prospect of using genetic engineering to modify fermentation reactions in order to improve photosynthetic hydrogen production in biotechnology.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element HYDROGEN PHOTOPRODUCTION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SULFUR DEPRIVATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element FERMENTATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PYRUVATE FORMATE LYASE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element HYDROGENASE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CHLAMYDOMONAS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Volgusheva, A. A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Petrova, E. V.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kukarskikh, G. P.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Dubini, A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Antal, T. K.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1YaW-GClVfwFY--luAjV9DVykI-ao_YNr/view?usp=drivesdk">https://drive.google.com/file/d/1YaW-GClVfwFY--luAjV9DVykI-ao_YNr/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-20603 25.06.2025 25.06.2025 Documentos solicitados