High-Performance Thin-Layer Chromatography hyphenated with image processing and chemometrics as a tool for forensic discrimination of Cannabis sativa L. chemotypes (Record no. 54702)

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003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625162452.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-20616
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245 10 - TITLE STATEMENT
Title High-Performance Thin-Layer Chromatography hyphenated with image processing and chemometrics as a tool for forensic discrimination of Cannabis sativa L. chemotypes
490 0# - SERIES STATEMENT
Volume/sequential designation Forensic Chemistry, 36, 100528, 2023
520 3# - SUMMARY, ETC.
Summary, etc. Cannabis sativa L. is the most widely cultivated, trafficked, and abused illicit drug. The reliable distinction between drug type and fiber type C. sativa remains a topic of interest for forensic chemists. For the first time, we applied a combination of simple, cost-effective, and reliable High-Performance Thin-Layer Chromatography (HPTLC)with image analysis as well as sophisticated multivariate tools for differentiating C. sativa drug, fiber and intermediate chemotypes. After extraction, major cannabinoids from 43 seized C. sativa were separated using the HPTLC method. Peak areas of ?9-THC, CBN, and CBD were calculated by simple image processing, and Xfactor?=?[THC?+?CBN]/CBD was determined as criteria used to discriminate three C. sativa chemotypes. The obtained results were compared with Gas Chromatography with Flame Ionization Detection (GC-FID)as the reference method for the determination of Xfactor. Principal Component Analysis and Hierarchic Cluster Analysis were applied to classify three C. sativa chemotypes according to their chemical pattern. The proposed approach clearly distinguishes 26 drugs, 13 fiber, and 4 intermediate chemotypes in seized C. sativa, aligning with GC-FID analysis. The results showed that the HPTLC technique in combination with multivariate methods is an accurate and reliable tool for high-throughput and forensic screening of three C. sativa chemotypes. Compounds such as ?9-THC and CBD were marked as the most important cannabinoids responsible for the classification of the seized C. sativa chemotypes.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CANNABIS SATIVA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element HIGH-PERFORMANCE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element THIN-LAYER
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CHROMATOGRAPHY XFACTOR
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element IMAGE ANALYSIS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CHEMOMETRICS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Radosavljevic-Stevanovic, N.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kovacevic, A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Manojlovic, D.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Ristivojevic, P.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1yYEmMkmzoGf8f3QljiWwtLtHoVzDFr0y/view?usp=drivesdk">https://drive.google.com/file/d/1yYEmMkmzoGf8f3QljiWwtLtHoVzDFr0y/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-20616 25.06.2025 25.06.2025 Documentos solicitados