Genome-wide characterization of NBS-LRR family genes and expression analysis under powdery mildew stress in Lagenaria siceraria (Record no. 54712)

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fixed length control field 02407nam a2200301Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625162452.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-20626
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245 10 - TITLE STATEMENT
Title Genome-wide characterization of NBS-LRR family genes and expression analysis under powdery mildew stress in Lagenaria siceraria
490 0# - SERIES STATEMENT
Volume/sequential designation Physiological and Molecular Plant Pathology, 118, p.101798, 2022
520 3# - SUMMARY, ETC.
Summary, etc. As the largest group of plant resistance genes, the nucleotide-binding site-leucine-rich repeat (NBS-LRR)genes play a critical role in plant disease defense system. The bottle gourd is a distinctive horticultural crop of the Cucurbitaceae family and powdery mildew is one of the major diseases jeopardizing crop production and quality. In this study, we have identified 84 NBS-LRR genes and divided into seven subfamilies. Phylogenetic tree and motif analysis grouped these genes into two major clusters with toll/interleukin-1 like receptor (TIR)domain or a coiled-coil domain. Subsequently, duplication events, motif sequence, orthologous gene cluster, protein-protein interaction, cis-elements were analyzed. 12 pairs of tandem duplicated genes and two pairs of segmental duplicated genes were detected in NBS-LRR family. 14 singleton genes and 62 functional cis-elements were found. The structure of 27 proteins were constructed by homology modeling and divided into five different types. 34 differentially expressed genes of NBS-LRR genes were found in PM-resistant and PM-susceptible line. Expression of NBS-LRR genes were validated by qRT-PCR under PM stress and gene Lsi04g015960 with powdery mildew8 (RPW8)domain was significantly up-regulated in PM-resistant variety. Our study provides fundamental insights of NBS-LRR family genes in bottle gourd and find a potential candidate gene Lsi04g015960 for PM tolerance breeding.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element BOTTLE GOURD
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element NBS-LRR FAMILY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PHYLOGENETIC ANALYSIS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element POWDERY MILDEW
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element EXPRESSION ANALYSIS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Wang, J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Yang, C.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Wu, X.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Wang, Y.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Wang, B.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Wu, X.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Li, G.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1Qza84hGz1tMg2ATYTDI_YLswWCe-cfRc/view?usp=drivesdk">https://drive.google.com/file/d/1Qza84hGz1tMg2ATYTDI_YLswWCe-cfRc/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-20626 25.06.2025 25.06.2025 Documentos solicitados