MARC details
| 000 -LEADER |
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| fixed length control field |
01943nam a2200349Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625162452.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-20630 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Creation of cardiac tissue exhibiting mechanical integration of spheroids using 3D bioprinting |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
JoVE (Journal of Visualized Experiments), 125, p.e55438, 2017 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
This protocol describes 3D bioprinting of cardiac tissue without the use of biomaterials, using only cells. Cardiomyocytes, endothelial cells and fibroblasts are first isolated, counted and mixed at desired cell ratios. They are co-cultured in individual wells in ultra-low attachment 96-well plates. Within 3 days, beating spheroids form. These spheroids are then picked up by a nozzle using vacuum suction and assembled on a needle array using a 3D bioprinter. The spheroids are then allowed to fuse on the needle array. Three days after 3D bioprinting, the spheroids are removed as an intact patch, which is already spontaneously beating. 3D bioprinted cardiac patches exhibit mechanical integration of component spheroids and are highly promising in cardiac tissue regeneration and as 3D models of heart disease. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
BIOENGINEERING |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ISSUE 125 |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CARDIAC TISSUE ENGINEERING |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
3D PRINTING |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
BIOPRINTING |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
BIOFABRICATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ADDITIVE MANUFACTURING |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
HEART FAILURE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
HUMAN INDUCED PLURIPOTENT STEM CELL-DERIVED CARDIOMYOCYTES |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Ong, C. S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Fukunishi, T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Nashed, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Blazeski, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Zhang, H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Hardy, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Hibino, N. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1bwsVTrrtRzwL0f0wCrqbz8gdS-q4fj3s/view?usp=drivesdk">https://drive.google.com/file/d/1bwsVTrrtRzwL0f0wCrqbz8gdS-q4fj3s/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
