Agrobacterium tumefaciens-Mediated Plant Transformation: A Review (Record no. 54758)

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control field MX-MdCICY
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control field 20250625162453.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-20672
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Title Agrobacterium tumefaciens-Mediated Plant Transformation: A Review
490 0# - SERIES STATEMENT
Volume/sequential designation Molecular Biotechnology, https://doi.org/10.1007/s12033-023-00788-x, 2023
520 3# - SUMMARY, ETC.
Summary, etc. Agrobacterium tumefaciens-mediated plant transformation is the most dominant technique for the transformation of plants. It is used to transform monocotyledonous and dicotyledonous plants. A. tumefaciens apply for stable and transient transformation, random and targeted integration of foreign genes, as well as genome editing of plants. The Advantages of this method include cheapness, uncomplicated operation, high reproducibility, a low copy number of integrated transgenes, and the possibility of transferring larger DNA fragments. Engineered endonucleases such as CRISPR/Cas9 systems, TALENs, and ZFNs can be delivered with this method. Nowadays, Agrobacterium-mediated transformation is used for the Knock in, Knock down, and Knock out of genes. The transformation effectiveness of this method is not always desirable. Researchers applied various strategies to improve the effectiveness of this method. Here, a general overview of the characteristics and mechanism of gene transfer with Agrobacterium is presented. Advantages, updated data on the factors involved in optimizing this method, and other useful materials that lead to maximum exploitation as well as overcoming obstacles of this method are discussed. Moreover, the application of this method in the generation of genetically edited plants is stated. This review can help researchers to establish a rapid and highly effective Agrobacterium-mediated transformation protocol for any plant species.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ENGINEERED ENDONUCLEASES
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Topical term or geographic name entry element GENOME EDITING
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TRANSGENIC PLANTS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TRANSIENT AND STABLE EXPRESSION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PLANT TRANSFORMATION
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Azizi-Dargahlou, S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Pouresmaeil, M.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1ypBAc2hYqDwMqdpmj2s_SajZWyCznkUc/view?usp=drivesdk">https://drive.google.com/file/d/1ypBAc2hYqDwMqdpmj2s_SajZWyCznkUc/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-20672 25.06.2025 25.06.2025 Documentos solicitados