MARC details
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02255nam a2200265Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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20250625162453.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-20700 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Cloning and characterization of a novel carotenoid cleavage dioxygenase 1 from Helianthus annuus |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Chemistry & BioDiversity, 19(1), p.e202100694, 2022 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Natural ?-ionone, a high-value flavoring agent, has been widely applied in the food, cosmetics, and perfume industry. However, attempts to overproduce ?-ionone in microorganisms have been limited by the efficiency of carotenoid cleavage dioxygenases (CCDs), which catalyzes ?-carotene in the biosynthesis pathway. In order to obtain CCD genes responsible for the specific cleavage of carotenoids generating ?-ionone, a novel carotenoid cleavage dioxygenase 1 from Helianthus annuus was cloned and overexpressed in Escherichia coli BL21(DE3). The recombinant CCD was able to cleave a variety of carotenoids at the 9, 10 (9', 10')sites to produce C13 products in vitro, including ?-ionone, pseudoionone, 3-hydroxy-4-oxo-?-ionone, 3-hydroxy-?-ionone, and 3-hydroxy-?-ionone, which vary depending on the carotenoid substrates. In comparison with lycopene and zeaxanthin, HaCCD1 also showed the high specificity for ?-carotene to cleave the 9, 10 (9', 10')double bond to produce ?-ionone in E. coli accumulating carotenoids. Finally, the expression of HaCCD1 in E. coli was optimized, and biochemical characterizations were further clarified. The optimal activity of HaCCD1 was at pH 8.8 and 50 °C. The Vmax for ?-apo-8'-carotenal was 10.14 U/mg, while the Km was 0.32 mM. Collectively, our study provides a valuable enzyme for the synthesis of natural ?-ionone by biotransformation and synthetic biology platform. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CCD1 |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
HELIANTHUS ANNUUS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CAROTENOID CLEAVAGE DIOXYGENASES |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
B-IONONE |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Qi, Z. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Tong, X. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Bu, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Pei, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Zhao, L. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1hVyIrfWUS8oAvvFGA9iEz0mTeUaLxm23/view?usp=drivesdk">https://drive.google.com/file/d/1hVyIrfWUS8oAvvFGA9iEz0mTeUaLxm23/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
