MARC details
| 000 -LEADER |
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| fixed length control field |
01818nam a2200241Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625162454.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-20712 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Heterologous expression and characterization of Penicillium citrinum nuclease P1 in Aspergillus niger and its application in the production of nucleotides |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Protein Expression and Purification, 156, p.36-43, 2019 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Nuclease P1 gene (nuc P1)which was cloned from Penicillium citrinum and expressed in A percent niger Bdel4 with the low-background extracellular protein percent The expression strategy of multi-copy nuc P1 in the A percent niger with the linker of 2A peptide was applied to improve the enzyme activity of nuclease P1, the highest activity up to 77 percent6 U/mL percent After Ni-chelate purification, the specific enzyme activity, the optimum temperature and pH were 32 percent4 U/mg, 65 °C and 5 percent3 respectively percent The recombination nuclease P1 was activated by addition of Mg2+, Zn2+ and Cu2+, and inhibited by addition of Ca2+, Fe2+, Mn2+, Ni2+, Co2+, Mg2+, K+ and EDTA percent Furthermore, the enzyme hydrolyses yeast RNA efficiently into 5'- nucleotides percent Through enzymolysis, the highest concentration of nucleotides achieved 15 percent12 mg/mL, and 75U nuclease P1 is suitable amount should be added to the enzymolysis system percent |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
NUCLEASE P1 |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
2A PEPTIDE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ASPERGILLUS NIGER |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
RECOMBINANT EXPRESSION |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Chen, X. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Wang, B. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Pan, L. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1cdWXN6i5MW7SVTdg7BpPxfXHslcZx-zd/view?usp=drivesdk">https://drive.google.com/file/d/1cdWXN6i5MW7SVTdg7BpPxfXHslcZx-zd/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
