MARC details
| 000 -LEADER |
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02345nam a2200313Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625162454.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-20747 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Influence of affinity tags and tobacco PR1a signal peptide on detection, purification and bioactivity analyses of the small oomycete apoplastic effectors |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Biotechnology Letters, 45(1), p.115-124, 2023 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Objective: To examine the influence of widely used protein affinity tags and the tobacco PR1a signal peptide (SP)on detection, purification and bioactivity analyses of the small oomycete apoplastic effector SCR96 in planta. Results: Through agroinfiltration, the phytotoxic effector SCR96 of Phytophthora cactorum was expressed in Nicotiana benthamiana leaf apoplast as a fusion protein carrying single affinity tag (His, HA or FLAG)at either C- or N-terminus. Leaf necrosis caused by different affinity-tagged SCR96 varied among tags and replicates. All of tagged proteins can be detected by antibodies against SCR96. All of SCR96 fusions except N-terminally fused 6His-tagged protein were detected using tag antibodies, indicating that 6His tag may be degraded when fused at N-terminus. Interestingly, C-terminal His- and FLAG-tagged SCR96 maintained the biological activity after purification. In the substitution assay of SCR96 SP, we observed that PR1a SP can lead chimeric SCR96 expression in N. benthamiana, but the replacement totally disrupted its bioactivity. Conclusion: C-terminal His or FLAG tag, along with its original SP, is efficient enough to enable detection and purification of functional SCR96 from N. benthamiana leaf apoplast, which would facilitate plant-pathogen interaction studies |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
AFFINITY TAG |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
AGROINFILTRATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
LEAF APOPLAST |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
PLANT CELL DEATH |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
SIGNAL PEPTIDE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
SMALL CYSTEINE-RICH EFFECTOR |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Jin, J. H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Zhou, X. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Liu, W. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Zhang, Z. H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Huang, S. X. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Zhao, W. J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Chen, X. R. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/192PIJvqRI4f5iMhSPN6-UEZPcnsZxHlS/view?usp=drivesdk">https://drive.google.com/file/d/192PIJvqRI4f5iMhSPN6-UEZPcnsZxHlS/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
