A restriction enzyme from Hemophilus influenzae: II. Base sequence of the recognition site (Record no. 54834)
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| fixed length control field | 01816nam a2200181Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625162454.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-20753 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | A restriction enzyme from Hemophilus influenzae: II. Base sequence of the recognition site |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Journal of Molecular Biology, 51(2), p.393-409, 1970 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Hemophilus influenzae strain Rd contains an enzyme, endonuolease R, which specifically degrades foreign DNA. With phage T7 DNA as substrate the endonuclease introduces a limited number (about 40)double-strand breaks (5?-phosphoryl, 3?-hydroxyl). The limit product has an average length of about 1000 nucleotide pairs and contains no single-strand breaks. We have explored the nucleotide sequences at the 5?-ends of the limit product by labeling the 5?- phosphoryl groups (using polynucleotide kinase)and characterizing the labeled fragments released by various nucleases. Two classes of 5?-terminal sequences were obtained: pApApCpNp . (60 percent)and pGpApCpNp . (40 percent), where N indicates that the base in the 4th position is not unique. The dinucleoside monophosphates at the 3?-ends were isolated after micrococcal nuclease digestion of the limit product and identified as TpT(60 percent)and TpC (40 percent). We conclude that endonuclease R of H. influenzae recognizes the following specific nucleotide sequence: 5? . pGpTpPy ¦pPupApCp . 3? 3? . pCpApPup ¦PypTpGp . 5? The implications of the twofold rotational symmetry of this sequence are discussed. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kelly Jr, T. J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Smith, H. O. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1F-5rnj_Lck5d8EbqYfpeTxB20srjhLo_/view?usp=drivesdk">https://drive.google.com/file/d/1F-5rnj_Lck5d8EbqYfpeTxB20srjhLo_/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-20753 | 25.06.2025 | 25.06.2025 | Documentos solicitados |