Regeneration of a transgenic woody legume (Robinia pseudoacacia L., black locust)and morphological alterations induced by Agrobacterium rhizogenes-mediated transformation (Record no. 54836)

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fixed length control field 01786nam a2200265Ia 4500
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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625162454.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-20755
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245 10 - TITLE STATEMENT
Title Regeneration of a transgenic woody legume (Robinia pseudoacacia L., black locust)and morphological alterations induced by Agrobacterium rhizogenes-mediated transformation
490 0# - SERIES STATEMENT
Volume/sequential designation Plant Science, 88(2), p.149-157, 1993
520 3# - SUMMARY, ETC.
Summary, etc. A repeatable and fast transformation-regeneration system has been developed for a woody legume, black locust (Robinia pseudoacacia L.). Hairy roots were produced from hypocotyl segments inoculated with Agrobacterium rhizogenes R1601, a super-virulent strain with eukaryotic kanamycin resistance gene, in less than 1 week and resulted in subsequent shoot regeneration within 4 weeks. Transgenic black locust trees were established in soil 12 weeks after inoculation with the A. rhizogenes strain. The hairy root phenotype found in regenerated plants, which has been characterized as having wrinkled leaves and abundant root development, became evident after 3 months of growth. Southern blot analysis clearly showed multiple integration of T-DNA (transferred DNA)and the presence of neomycin phosphotransferase (NPTII)gene sequences in the transformed black locust genome.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element AGROBACTERIUM
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element BLACK LOCUST
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element HAIRY ROOT
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element LEGUME
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TRANSFORMATION
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Han, K. H.,
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Keathley, D. E.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Davis, J. M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Gordon, M. P.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1fPVJVT2XjnoGuZGjzWS-HuEsvoTXk7vU/view?usp=drivesdk">https://drive.google.com/file/d/1fPVJVT2XjnoGuZGjzWS-HuEsvoTXk7vU/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-20755 25.06.2025 25.06.2025 Documentos solicitados