Effect of spermidine, glutamine, and proline on somatic embryogenesis and silver nanoparticles supplied culture improved rhizome formation of Panax vietnamensis var. langbianensis (Record no. 54854)

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003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625162455.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-20773
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245 10 - TITLE STATEMENT
Title Effect of spermidine, glutamine, and proline on somatic embryogenesis and silver nanoparticles supplied culture improved rhizome formation of Panax vietnamensis var. langbianensis
490 0# - SERIES STATEMENT
Volume/sequential designation South African Journal of Botany, 163, p.226-236, 2023
520 3# - SUMMARY, ETC.
Summary, etc. Lang Bian ginseng (Panax vietnamensis var. langbianensis)is a rare and traditional herbal medicine with many pharmacological activities. It was first discovered by Duy et al. (2016)in Lam Dong province, Vietnam. Until now, there has been only one publication on Lang Bian ginseng micropropagation via somatic embryogenesis (Anh et al., 2022). However, this study still had limitations, including the small number of somatic embryos and the time-consuming. This study investigated the effect of spermidine, glutamine, and proline on the enhancement of somatic embryogenesis (SE)of Lang Bian ginseng using thin cell layer culture technology. The results showed that the optimal SE rate and the number of somatic embryos per explant were achieved on MS medium supplemented with 0.01 mM spermidine in leaf explants (93.32 percent and 54.20 embryos, respectively)and petiole explants (96.66 percent and 68.80 embryos, respectively)and higher compared to these in other treatments, including control, glutamine, and proline treatments. Besides, adding 0.01 mM spermidine to the culture medium also improved the quality of somatic embryos, mainly cotyledonary stage, through an enhanced synthesis of antioxidant enzymes [catalase (CAT)and superoxide dismutase (SOD), and ascorbate peroxidase (APX)]. In addition, the fluctuations of endogenous hormones during initiation and maturation of SE derived from 0.01 mM spermidine treatment were recorded. Endogenous CKs (ZEA, 2iP, KIN, mT), IAA, and GA3 concentrations were the highest during the induction stage in both explants. Meanwhile, the remaining endogenous hormones (MEL, ABA, and SA)exhibited no inevitable fluctuation trends. In addition, IAA was only detected at the induction stage of SE in both explants. Moreover, cotyledonary somatic embryos derived from 0.01 mM spermidine treatment grew well in MS medium supplemented with 1.2 mg/L AgNPs.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element AMINO ACID
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ANTIOXIDANT ENZYME
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ENDOGENOUS HORMONE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element LANG BIANG INSENG
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element POLYAMINE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SOMATIC EMBRYO
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Anh, T. T. L.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Mai, N. T. N.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Tung, H. T.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Khai, H. D.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Luan, V. Q.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Phuong, H. T. N.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Nhut, D. T.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1WmNreQTE3qn7-08gAkIUkKhDYQdbBqCQ/view?usp=drivesdk">https://drive.google.com/file/d/1WmNreQTE3qn7-08gAkIUkKhDYQdbBqCQ/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-20773 25.06.2025 25.06.2025 Documentos solicitados