Somatic embryogenesis in Euterpe edulis Martius is improved by wounding, explant orientation, and suspension culture (Record no. 54856)

MARC details
000 -LEADER
fixed length control field 02600nam a2200325Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625162455.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-20775
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
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245 10 - TITLE STATEMENT
Title Somatic embryogenesis in Euterpe edulis Martius is improved by wounding, explant orientation, and suspension culture
490 0# - SERIES STATEMENT
Volume/sequential designation Plant Cell, Tissue and Organ Culture , 156(2), p.31, 2024
520 3# - SUMMARY, ETC.
Summary, etc. Illegal extraction of the heart of palm is threatening Euterpe edulis Martius with extinction. Here, we investigated the induction of somatic embryogenesis in segments of E. edulis seedlings as a means of propagating this palm species. Immature seeds were harvested from the wild and germinated in vitro. After 6 months, the seedlings were excised in the middle of the caulicle and cut either transversely into two explants, or longitudinally with the wounded surface face down, up or sideways on the medium, supplemented with picloram 150 µM. Friable calli formed from upward facing explants were transferred to a suspension culture with different concentrations of picloram (15, 25, 35, and 45 µM)and then matured in the presence of abscisic acid (1, 5, 10, and 20 µM). Explants derived from upward facing segments were placed in culture medium containing l-glutamine or hydrolyzed casein (0.0, 0.5, and 1.0 g L?1). Induction in medium with 150 µM picloram was strongest for stems with longitudinal wounds positioned upward and/or sideways; while medium with 15 µM picloram enabled strong growth of friable calli. The highest average number of proembryos (16.33)was obtained with 1.0 g L?1 hydrolyzed casein and differentiation of somatic embryos was greatest with 1 µM abscisic acid. Therefore, somatic embryogenesis of E. edulis is best achieved by placing segments from longitudinally wounded stems face up on medium containing 150 µM picloram, followed by suspension cultivation with 15 µM picloram and maturation with 1 µM abscisic acid.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element JUCARA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element IN VITRO CULTURE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element WOUNDING
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PICLORAM
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element L-GLUTAMINE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element HYDROLYZED CASEIN
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TOTIPOTENCY
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name De Mello, T.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Silva, T. D.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Zanardo, T. É. C.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name De Almeida, F. A. N.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Oliveira, L. B.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Hegedus, C. E. N.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Alexandre, R. S.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/14jV86KGKzmFom4qK4suly7C-d3SKeDex/view?usp=drivesdk">https://drive.google.com/file/d/14jV86KGKzmFom4qK4suly7C-d3SKeDex/view?usp=drivesdk</a>
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942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
Holdings
Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-20775 25.06.2025 25.06.2025 Documentos solicitados