Somatic embryogenesis and genetic homogeneity assessment of regenerated plants of Crinum brachynema (Amaryllidaceae): an endemic critically endangered medicinal plant (Record no. 54860)
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| fixed length control field | 02561nam a2200277Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625162455.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-20779 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Somatic embryogenesis and genetic homogeneity assessment of regenerated plants of Crinum brachynema (Amaryllidaceae): an endemic critically endangered medicinal plant |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Plant Cell, Tissue and Organ Culture, 156(2), p.33, 2024 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Crinum brachynema is a bulbous plant belonging to the family Amaryllidaceae which is restricted to Western India. Due to its high rarity and low distribution range, it has been classified as "critically endangered". Establishing an efficient and unprecedented somatic embryogenesis protocol is necessary for its conservation and large-scale propagation. In this study,regeneration was achieved through somatic embryogenesis using bulb explants on MS media supplemented with various concentrations of 2,4-D alone and in combination with N6-benzyl-adenine. Different advanced phases with maturation of somatic embryo were obtained on MS medium with different ratios of picloram and thidiazuron (TDZ). The highest number of somatic embryos (50.33 _}1.52)was obtained after eight weeks in the medium supplemented picloram (2.0 mg L?1)in combination with TDZ (0.5 mg L?1). MS medium with reduced concentration of salts in combination with GA3 (1.0 mg L?1)was used for somatic embryo germination. The maximum embryo germination frequency (82.22)was recorded on half-strength MS medium fortified with 1 mg L?1 GA3.The genetic true-to-typeness of regenerated plants was confirmed by ISSR, SCoT and RAPD primers based molecular analyses. This confirmed their genetic homogeneity compared to the mother plant and it also demonstrated the reliability of our somatic embryogenesis system for C. brachynema. The protocol developed may facilitate efforts in reintroduction, restoration, and ex situ conservation of C. brachynema in its natural hábitat and its potential commercial utilization. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CONSERVATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CRINUM BRACHYNEMA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CRITICALLY ENDANGERED |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | GENETIC FIDELITY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | MOLECULAR MARKERS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | SOMATIC EMBRYO |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kaur, H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Lekhak, M. M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ochatt, S. J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kumar, V. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1H0CewPN6GIgxgmX75p4tQqu8InWfv69F/view?usp=drivesdk">https://drive.google.com/file/d/1H0CewPN6GIgxgmX75p4tQqu8InWfv69F/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-20779 | 25.06.2025 | 25.06.2025 | Documentos solicitados |