Double-haploid plant production through anther and ovule culture of wild Cyclamen persicum Mill. and Melody F1 cyclamen cultivar (Record no. 54869)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 02699nam a2200277Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625162455.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-20788 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Double-haploid plant production through anther and ovule culture of wild Cyclamen persicum Mill. and Melody F1 cyclamen cultivar |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | In Vitro Cellular & Developmental Biology-Plant, 59(6), p.711-723, 2023 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | In vitro anther and ovule culture has been mostly used in haploidization studies of annual and perennial plants to shorten the process of breeding. Cyclamen genus is one of the major perennial geophytes widely used as an ornamental plant. The aim of this study was to develop an efficient haploid plant regeneration protocol via anther and ovule culture for wild Cyclamen persicum and commercial F1 Melody cultivar. The uninuclear stage of microspore was determined with DAPI (4?,6-diamidino-2-phenylindole dihydrochloride)dye for C. persicum Mill. and commercial cultivar. Cold pre-treatment (4 °C)was applied to the buds for two d before in vitro ovule and anther cultures. Anthers were cultured on B5 medium combined with different dosages of 1-naphthaleneacetic acid (NAA), 135.0 g L?1 maltose, silver nitrate (AgNO3), and activated charcoal (AC). Ovules were cultured on Murashige and Skoog (MS)medium including the varied amount of 2,4-dichlorophenoxyacetic acid (2,4-D)and N6-(2-isopentenyladenine)(2iP)and sucrose. Embryos were maturated and germinated on the M2 medium (MS containing 0.2 mg L?1 giberellic acid (GA3), 0.1 mg L?1 6-benzylaminopurine (BA), 1.0 g L?1 proline, and 0.05 mg L?1 spermine)for anther culture and MS medium without plant growth regulator (PGR)for ovule culture. Haploid embryos were obtained from B5 medium, including 1.0 mg L?1 NAA for C. persicum Mill. (100 percent). An efficient ovule culture protocol was determined for C. persicum Mill. as 2.0 mg L?1 2,4-D and 0.8 mg L?1 2iP; and 2.0 mg L?1 2,4-D and 0.5 mg L?1 2iP for Melody F1 cultivar as 100 percent. Spontaneous double haploidization was detected on C. persicum Mill. via flow cytometric analysis. Plants were transferred to the soil, and blooming was observed 4 mo after acclimatization. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | ANTHER |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | B5 |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CALLUS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | EMBRYO MS5OVULE |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sevindik, B. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Izgü, T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Tütüncü, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Çürük, P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sari, N. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mendi, Y. Y. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1I2un0SnHMUijeAcwE82xrYBEGhqUQTA8/view?usp=drivesdk">https://drive.google.com/file/d/1I2un0SnHMUijeAcwE82xrYBEGhqUQTA8/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-20788 | 25.06.2025 | 25.06.2025 | Documentos solicitados |