MARC details
| 000 -LEADER |
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| fixed length control field |
02718nam a2200325Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625162457.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-20870 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
A newly characterized dense granule protein (GRA76)is important for the growth and virulence of Toxoplasma gondii |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
International Journal for Parasitology, 54(2), p.109-121, 2024 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Pathogenicity of the zoonotic pathogen Toxoplasma gondii largely depends on the secretion of effector proteins into the extracellular milieu and host cell cytosol, including the dense granule proteins (GRAs). The protein-encoding gene TGME49_299780 was previously identified as a contributor to parasite fitness. However, its involvement in parasite growth, virulence and infectivity in vitro and in vivo remains unknown. Here, we comprehensively examined the role of this new protein, termed GRA76, in parasite pathogenicity. Subcellular localization revealed high expression of GRA76 in tachyzoites inside the parasitophorous vacuole (PV). However, its expression was significantly decreased in bradyzoites. A CRISPR-Cas9 approach was used to knock out the gra76 gene in the T. gondii type I RH strain and type II Pru strain. The in vitro plaque assays and intracellular replication showed the involvement of GRA76 in replication of RH and Pru strains. Deletion of the gra76 gene significantly decreased parasite virulence, and reduced the brain cyst burden in mice. Using RNA sequencing, we detected a significant increase in the expression of bradyzoite-associated genes such as BAG1 and LDH2 in the Pru?gra76 strain compared with the wild-type Pru strain. Using an in vitro bradyzoite differentiation assay, we showed that loss of GRA76 significantly increased the propensity for parasites to form bradyzoites. Immunization with Pru?gra76 conferred partial protection against acute and chronic infection in mice. These findings show the important role of GRA76 in the pathogenesis of T. gondii and highlight the potential of Pru?gra76 as a candidate for a live-attenuated vaccine. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
BRADYZOITE DIFFERENTIATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
DENSE GRANULE PROTEINS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
GRA76 |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
REPLICATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
TOXOPLASMA GONDII |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
VACCINE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
VIRULENCE |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Zheng, X. N. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Sun, L. X. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Elsheikha, H. M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Li, T. T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Gao, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Wu, X. J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Wang, J. L. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1Wmjju7roG8NGy0AgrIwHLcFZWGFzEB17/view?usp=drivesdk">https://drive.google.com/file/d/1Wmjju7roG8NGy0AgrIwHLcFZWGFzEB17/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
