Cloning and expression analysis of a nitrate reductase gene related to somatic embryogenesis in Gossypium hirsutum (Record no. 54964)
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| fixed length control field | 01821nam a2200289Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625162457.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-20884 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Cloning and expression analysis of a nitrate reductase gene related to somatic embryogenesis in Gossypium hirsutum |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Scientific Research and Essays, 7(3), p.300-309, 2012 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Nitrate reductase is a key enzyme in the overall process of nitrate assimilation by plants. A nitrate reductase (NR)gene related to somatic embryogenesis was firstly cloned from Gossypium hirsutum. Sequence alignment shows that the GhNR is closest with Tilia platyphyllos NR gene. The expression of GhNR was investigated using semi-quantitative RT-PCR in nonembryogenic calli, embryogenic calli and somatic embryoids which showed that the expression level of GhNR was higher in embryogenic calli and somatic embryoids than in nonembryogenic calli. Besides, the expression of GhNR gene was verified by transformation in E. coli BL21 (DE3)strain with the recombinant expression vector pET-32a. NR activity assay showed that the crude GhNR protein had obvious activity to KNO3 substrate. The results implied that NR has a critical function in the assimilation of nitrogen in cotton somatic embryo germination. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | COTTON |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | SOMATIC EMBRYOGENESIS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | NITRATE REDUCTASE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | GENE EXPRESSION |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Liu, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Han, G. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zhang, Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wang, X. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Chi, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zhang, G. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ma, Z. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1LrVsXtX0vpe4djs2XL2N7pdBrV9QIAlt/view?usp=drivesdk">https://drive.google.com/file/d/1LrVsXtX0vpe4djs2XL2N7pdBrV9QIAlt/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-20884 | 25.06.2025 | 25.06.2025 | Documentos solicitados |