Genetic stability analysis of the temporary immersion bioreactors-derived sugarcane seedlings with simple sequence repeat (SSR)markers (Record no. 54966)

MARC details
000 -LEADER
fixed length control field 02658nam a2200289Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625162457.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-20886
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 250602s9999 xx |||||s2 |||| ||und|d
245 10 - TITLE STATEMENT
Title Genetic stability analysis of the temporary immersion bioreactors-derived sugarcane seedlings with simple sequence repeat (SSR)markers
490 0# - SERIES STATEMENT
Volume/sequential designation Plant Cell, Tissue and Organ Culture, 156(1), p.22, 2024
520 3# - SUMMARY, ETC.
Summary, etc. The utilization of temporary immersion system-bioreactors (TIB)has shown promise for sugarcane micropropagation due to its capacity for high-yield seedlings production. However, the temporary immersion system also carries the risk of somaclonal variations (SV)in regenerated plants. SV, encompassing phenotypic changes resulting from genetic or epigenetic alterations, and it presents challenges in preserving the genetic uniformity of the seedlings, a crucial factor for sugarcane productivity. A molecular marker like Simple Sequence Repeats (SSR)offers a reliable means to assess the genetic stability of TIB-derived sugarcane seedlings, ensurinng that the temporary immersion system-based micropropagation produces true-to-type planting materials. In this study, SSR analysis was conducted to determine the relative genetic stability of TIB-derived sugarcanes, using 15 SSR markers, generating over 200 DNA fragments ranging from 150 to 4,000 bp in size. The genetic stability was calculated as the Jaccard's similarity index of the SSR patterns of TIB-derived sugarcanes compared to their mother plants. The genetic stability varied between 92 and 96 percent in three Indonesian sugarcane varieties (PSKA 942, PS 094, and PS 091)which is relatively high considering the high cell mass multiplication in TIB (up to 30 multiplies). Morphological observations in the field also revealed no differences between TIB-derived sugarcane and their mother plants, convincing the true-to-type of micropropagation products. This study demonstrated the high relative genetic stability of TIB-derived sugarcanes, paving the way for large-scale commercial application in plantations.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GENETIC STABILITY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SSR
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SUGARCANE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TEMPORARY IMMERSION BIOREACTOR (TIB)
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Saptari, R. T.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Aksa, A. A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Riyadi, I.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Prasetyo, M. E. R. B.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Lindawati, S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Setiawati, Y.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Sumaryono, S
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/17dRp9dQal-9lDJd5U55OgYzGDXqLVC9w/view?usp=drivesdk">https://drive.google.com/file/d/17dRp9dQal-9lDJd5U55OgYzGDXqLVC9w/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-20886 25.06.2025 25.06.2025 Documentos solicitados