MARC details
| 000 -LEADER |
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| fixed length control field |
02823nam a2200313Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625162458.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-20943 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Seed-derived defensins from Scots pine: structural and functional features |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Planta, 254, p.1-18, 2021 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
The recombinant PsDef5.1 defensin inhibits the growth of phytopathogenic fungi, Gram-positive and Gram-negative bacteria, and human pathogen Candida albicans. Expression of seed-derived Scots pine defensins is tissue-specific and developmentally regulated. Plant defensins are ubiquitous antimicrobial peptides that possess a broad spectrum of activities and multi-functionality. The genes for these antimicrobial proteins form a multigenic family in the plant genome and are expressed in every organ. Most of the known defensins have been isolated from seeds of various monocot and dicot species, but seed-derived defensins have not yet been characterized in gymnosperms. This study presents the isolation of two new 249 bp cDNA sequences from Scots pine seeds with 97.9 percent nucleotide homology named PsDef5.1 and PsDef5.2. Their deduced amino acid sequences have typical plant defensin features, including an endoplasmic reticulum signal sequence of 31 amino acids (aa), followed by a characteristic defensin domain of 51 aa. To elucidate the functional activity of new defensins, we expressed the mature form of PsDef5.1 in a prokaryotic system. The purified recombinant peptide exhibited activity against the phytopathogenic fungi and Gram-negative and Gram-positive bacteria with the IC50 of 5-18 µM. Moreover, it inhibited the growth of the human pathogen Candida albicans with the IC50 of 6.0 µM. Expression analysis showed that transcripts of PsDef5.1-2 genes were present in immature and mature pine seeds and different parts of seedlings at the early stage of germination. In addition, unlike the PsDef5.2, the PsDef5.1 gene was expressed in the reproductive organs. Our findings indicate that novel defensins are promising candidates for transgenic application and the development of new antimicrobial drugs. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ANTIFUNGAL AND ANTIBACTERIAL ACTIVITIES |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ANTIMICROBIAL PEPTIDE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CLONING |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
EXPRESSION PATTERN |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
HETEROLOGICAL EXPRESSION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
PINUS SYLVESTRIS L |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Shalovylo, Y. I. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Yusypovych, Y. M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Hrunyk, N. I. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Roman, I. I. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Zaika, V. K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Krynytskyy, H. T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Kovaleva, V. A. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1mV4UiCk_Ifb7adJIIJA0m8uN1H8sn5vd/view?usp=drivesdk">https://drive.google.com/file/d/1mV4UiCk_Ifb7adJIIJA0m8uN1H8sn5vd/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
