MARC details
| 000 -LEADER |
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| fixed length control field |
02569nam a2200289Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625162458.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-20944 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Gene isolation and structural characterization of a legume tree defensin with a broad spectrum of antimicrobial activity |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Planta, 250, p.1757-1772, 2019 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
The recombinant EcgDf1 defensin has an antimicrobial effect against both plant and human pathogens. In silico analyses predict that EcgDf1 is prone to form dimers capable of interacting with the membranes of microorganisms. Plant defensins comprise a large family of antimicrobial peptides (AMP)with a wide range of biological functions. They are cysteine-rich molecules, highly sequence diverse but with a conserved and stable structure. In this work, a defensin gene (EcgDf1)was isolated from Erythrina crista-galli, a legume tree native from South America. The predicted peptide presents eight cysteines, with a ?-core motif GXCX3-9C and six cysteines distributed like the typical defensin ?? motif. The mature EcgDf1 coding sequence was heterologously expressed in Escherichia coli strains and purified by affinity chromatography. Possible dimer and oligomers of EcgDf1 were visible in SDS electrophoresis. Moreover, its 3D structure, determined by homology modeling, docking, and molecular dynamics simulations, was found to be compatible with the formation of homodimers between the ?3 and ?1-loop-?1, leaving the ?2-loop-?3 free to interact with lipid membranes. The purified recombinant peptide inhibited the growth of several critical plant and human pathogens, like the opportunistic fungi Candida albicans and Aspergillus niger and the plant pathogens Clavibacter michiganensis ssp. michiganensis, Penicillium expansum, Botrytis cinerea, and Alternaria alternata. EcgDf1 is a promising candidate for the development of antimicrobial products for use in agriculture and medicine. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ANTIMICROBIAL PEPTIDES |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
DIMERIZATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
DOCKING |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ERYTHRINA CRISTA-GALLI |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
HETEROLOGOUS EXPRESSION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
MOLECULAR DYNAMICS SIMULATION |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Rodríguez-Decuadro, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Dans, P. D. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Borba, M. A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Benko-Iseppon, A. M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Cecchetto, G. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/12021DkUXQaPm351DGPvIWJ8T38kqlmCP/view?usp=drivesdk">https://drive.google.com/file/d/12021DkUXQaPm351DGPvIWJ8T38kqlmCP/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
