MARC details
| 000 -LEADER |
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| fixed length control field |
02572nam a2200313Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625162458.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-20952 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Isolation, culture of protoplasts of Angelica gigas Nakai and regeneration of plants via somatic embryogenesis |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Plant Cell, Tissue and Organ Culture, 156(2), p.40, 2024 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
In plant biotechnology, protoplasts are a versatile tool since they are very helpful for both fundamental biology studies and for genetic improvement and genome editing studies. In many plant species, however, reproducible regeneration from protoplasts continues to be a bottleneck. In the present study, we report the development of an efficient method for protoplast isolation, and plant regeneration in Angelica gigas via indirect somatic embryogenesis. Protoplasts were isolated from embryogenic callus using an enzyme mixture of 1.0 percent Viscozyme® L?+?1 percent Celluclast® 1.5 L?+?0.5 percent Pectinex® XXL with 7 h treatments. Initially, protoplasts were cultured in MS, modified MS (NH4NO3-free medium), and KM media, and viability and cell division data showed the MS medium was suitable for protoplast culture. Subsequently, the thin alginate layer method was applied to the protoplast culture at an optimal density of 1?×?106 cells per mL??1 and verified the effect of 2,4-D (0.1, 0.5, and 1.0 mg L??1)alone, and 2,4-D (0.5, and 1.0 mg L??1)in combination with BA (0.1 and 0.5 mg L??1)or KN (0.1 and 0.5 mg L??1)on cell division, micro-callus formation. MS medium supplemented with 1.0 mg L??1 2,4-D and 0.1 mg L??1 KN induced optimal cell division, callus formation, and subsequent induction of somatic embryogenesis from the callus. The somatic embryos germinated and converted into plantlets upon transferring to the MS basal medium. This method of Angelica gigas protoplast regeneration can be used for the genetic improvement of this plant. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ANGELICA GIGAS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
PROTOPLAST |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
PLANT GROWTH REGULATORS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
PLANT REGENERATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
SOMATIC EMBRYOGENESIS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
TISSUE CULTURE |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Lee, H. S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Han, J. E. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Jie, E. Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Kim, S. W. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Kwon, H. J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Lee, G. M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Park, S. Y. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1URgtWEMpLSF2QKuVSvfH3dkU8pR4GKDb/view?usp=drivesdk">https://drive.google.com/file/d/1URgtWEMpLSF2QKuVSvfH3dkU8pR4GKDb/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
