Hydrolytic endonucleolytic ribozyme (HYER)is programmable for sequence-specific DNA cleavage (Record no. 55055)
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| fixed length control field | 01789nam a2200241Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625162459.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-20976 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Hydrolytic endonucleolytic ribozyme (HYER)is programmable for sequence-specific DNA cleavage |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Science, 383(6682), p.eadh4859, 2024 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Ribozymes are catalytic RNAs with diverse functions including self-splicing and polymerization. This work aims to discover natural ribozymes that behave as hydrolytic and sequence-specific DNA endonucleases, which could be repurposed as DNA manipulation tools. Focused on bacterial group II-C introns, we found that many systems without intron-encoded protein propagate multiple copies in their resident genomes. These introns, named HYdrolytic Endonucleolytic Ribozymes (HYERs), cleaved RNA, single-stranded DNA, bubbled double-stranded DNA (dsDNA), and plasmids in vitro. HYER1 generated dsDNA breaks in the mammalian genome. Cryo-electron microscopy analysis revealed a homodimer structure for HYER1, where each monomer contains a Mg2+-dependent hydrolysis pocket and captures DNA complementary to the target recognition site (TRS). Rational designs including TRS extension, recruiting sequence insertion, and heterodimerization yielded engineered HYERs showing improved specificity and flexibility for DNA manipulation. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Liu, Z. X. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zhang, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zhu, H. Z. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Chen, Z. H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Yang, Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Li, L. Q. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Liu, J. J. G. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1jcc4PS2QyAsI8_GS0BM41Q0INR7ICdlz/view?usp=drivesdk">https://drive.google.com/file/d/1jcc4PS2QyAsI8_GS0BM41Q0INR7ICdlz/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-20976 | 25.06.2025 | 25.06.2025 | Documentos solicitados |