A rapid and robust colorimetric method for measuring relative abundance of auxins in plant tissues (Record no. 55056)
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| fixed length control field | 02588nam a2200229Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625162459.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-20977 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | A rapid and robust colorimetric method for measuring relative abundance of auxins in plant tissues |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | PhytoChemical Analysis, 10.1002/pca.3340, 2024 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Introduction: Auxin estimation in plant tissues is a crucial component of auxin signaling studies. Despite the availability of various high-throughput auxin quantification methods like LC-MS, GC-MS, HPLC, biosensors, and DR5-gus/gfp-based assays, auxin quantification remains troublesome because these techniques are very expensive and technology intensive and they mostly involve elaborate sample preparation or require the development of transgenic plants. Objectives: To find a solution to these problems, we made use of an old auxin detection system to quantify microbe derived auxins and modified it to effectively measure auxin levels in rice plants. Materials and methods: Auxins from different tissues of rice plants, including root samples of seedlings exposed to IAA/TIBA or subjected to different abiotic stresses, were extracted in ethanol. The total auxin level was measured by the presently described colorimetric assay and counterchecked by other auxin estimation methods like LC-MS or gus staining of DR5-gus overexpressing lines. Results: The presented colorimetric method could measure (1)the auxin levels in different tissues of rice plants, thus identifying the regions of higher auxin abundance, (2)the differential accumulation of auxins in rice roots when auxin or its transport inhibitor was supplied exogenously, and (3)the levels of auxin in roots of rice seedlings subjected to various abiotic stresses. The thus obtained auxin levels correlated well with the auxin levels determined by other methods like LC-MS or gus staining and the expression pattern of auxin biosynthesis pathway genes. Conclusions: The auxin estimation method described here is simple, rapid, cost-effective, and sensitive and allows for the efficient detection of relative auxin abundances in plant tissues. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | AUXIN ESTIMATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PLANT TISSUE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | RAPID AND ROBUST METHOD |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Manna, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Rengasamy, B. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sinha, A. K. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1ZyPXNmrMVibgGos2TLnPj9Y2LQ1byb9D/view?usp=drivesdk">https://drive.google.com/file/d/1ZyPXNmrMVibgGos2TLnPj9Y2LQ1byb9D/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-20977 | 25.06.2025 | 25.06.2025 | Documentos solicitados |