A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide (Record no. 55109)

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control field 20250625162500.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-21031
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Title A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide
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Volume/sequential designation Nature Protocols, 1(5), p.2320-2325, 2006
520 3# - SUMMARY, ETC.
Summary, etc. We describe a modification of the DNA extraction method, in which cetyltrimethylammonium bromide (CTAB)is used to extract nucleic acids from plant tissues. In contrast to the original method, the modified CTAB procedure is faster, omits the selective precipitation and CsCl gradient steps, uses less expensive and toxic reagents, requires only inexpensive laboratory equipment and is more readily adapted to high-throughput DNA extraction. This protocol yields approximately 5-30 microg of total DNA from 200 mg of tissue fresh weight, depending on plant species and tissue source. It can be completed in as little as 5-6 h.
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Personal name Allen, G. C.
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Personal name Flores-Vergara, M. A.
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Personal name Krasynanski, S.
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Personal name Kumar, S.
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Personal name Thompson, W. F.
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Uniform Resource Identifier <a href="https://drive.google.com/open?id=14_7dRgCRZ4Io5f7f6tByssGoR2C84fSj&usp=drive_copy">https://drive.google.com/open?id=14_7dRgCRZ4Io5f7f6tByssGoR2C84fSj&usp=drive_copy</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-21031 25.06.2025 25.06.2025 Documentos solicitados