Characterization of the cAMP phosphodiesterase domain in plant adenylyl cyclase/cAMP phosphodiesterase CAPE from the liverwort Marchantia polymorpha. (Record no. 55127)
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| fixed length control field | 01865nam a2200265Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625162500.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-21049 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Characterization of the cAMP phosphodiesterase domain in plant adenylyl cyclase/cAMP phosphodiesterase CAPE from the liverwort Marchantia polymorpha. |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Journal of Plant Research, 135, p.137-144, 2022 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Cyclic AMP (cAMP)acts as a second messenger and is involved in the regulation of various physiological responses. Recently, we identified the cAMP-synthesis/hydrolysis enzyme CAPE, which contains the two catalytic domains adenylyl cyclase (AC)and cAMP phosphodiesterase (PDE)from the liverwort Marchantia polymorpha. Here we characterize the PDE domain of M. polymorpha CAPE (MpCAPE-PDE)using the purified protein expressed in E. coli. The Km and Vmax of MpCAPE-PDE were 30 µM and 5.8 nmol min-1 mg-1, respectively. Further, we investigated the effect of divalent cations on PDE activity and found that Ca2+ enhanced PDE activity, suggesting that Ca2+ may be involved in cAMP signaling through the regulation of PDE activity of CAPE. Among the PDE inhibitors tested, only dipyridamole moderately inhibited PDE activity by approximately 40 percent at high concentrations. Conversely, 3-isobutyl-1-methylxanthine (IBMX)did not inhibit PDE activity. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | ADENYLYL CYCLASE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CAPE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | MARCHANTIA POLYMORPHA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PHOSPHODIESTERASECAMP |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hayashida, Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Yamamoto, C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Takahashi, F. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Shibata, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kasahara, M |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/open?id=1p_9wsJJsjI89jCXTeCPrfFiLxw0HEcYB&usp=drive_copy">https://drive.google.com/open?id=1p_9wsJJsjI89jCXTeCPrfFiLxw0HEcYB&usp=drive_copy</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-21049 | 25.06.2025 | 25.06.2025 | Documentos solicitados |