Phycocyanin-conjugated chitosan-coated iron oxide nanoparticles for the separation of Escherichia coli cells (Record no. 55169)
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| fixed length control field | 03120nam a2200301Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625162501.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-21091 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Phycocyanin-conjugated chitosan-coated iron oxide nanoparticles for the separation of Escherichia coli cells |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Biomass Conversion and Biorefinery, https://doi.org/10.1007/s13399-023-04529-7, 2023 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | This study proposes a novel method for isolating Escherichia coli bacteria using iron oxide nanoparticles coated with phycocyanin, a fluorescent blue protein pigment found in cyanobacteria that can bind to the genomic area of the cell. This novel technique can be a promising method for the removal of E. coli cells from polluted water samples and also in the downstream processing sector with the separation of E. coli cells using phycocyanin-conjugated chitosan-coated iron oxide nanoparticles with high efficiency. Moreover, the conjugate can also be used for rapid and quick separation and detection of polluted samples. Hence, this study introduces a novel application of phycocyanin as a cell separation method, utilizing its binding affinity to effectively attach to microbial cells for separation purposes. Phycocyanin pigment was extracted from Spirulina platensis, which was later purified using activated charcoal. A yield of 17.6 mg/g of biomass was obtained along with a purity index of 1.2 from the algal biomass. Chitosan-coated magnetic iron oxide nanoparticles were synthesized using the coprecipitation method. The synthesized magnetic nanoparticles were characterized using various techniques such as field emission scanning electron microscopy (FE-SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and zeta potential. The results showed that synthesized nanoparticles were 12-15 nm in size. The E. coli cells associated with the phycocyanin-conjugated nanoparticles were separated using a neodymium magnet due to the magnetic property of nanoparticles. The optimum concentration of phycocyanin-conjugated chitosan-coated nanoparticles was found to be 400 mg/L for maximum separation efficiency. The separation effectiveness was found to be 65.7 percent based on measurements of cell density before and after separation. The attachment of cells to the nanoparticles was observed using fluorescence and transmission electron microscopy. Thus, the coating of phycocyanin over chitosan magnetic nanoparticles allows the adhesion of E. coli cells onto the nanoparticles. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CHITOSAN |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | IRON OXIDE NANOPARTICLES |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PHYCOCYANIN |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | E. COLI |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | FLUORESCENCE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CELL DENSITY |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Nair, M. S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Rajarathinam, R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Velmurugan, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Devakumar, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Karthikayan, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Saravanakumar, S. P. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/open?id=16Skqrlme8EWGoZmdswOw4lOmC6QUnk3S&usp=drive_copy">https://drive.google.com/open?id=16Skqrlme8EWGoZmdswOw4lOmC6QUnk3S&usp=drive_copy</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-21091 | 25.06.2025 | 25.06.2025 | Documentos solicitados |