MARC details
| 000 -LEADER |
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02950nam a2200301Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625164351.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-21217 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
The chitin-binding domain of Bacillus thuringiensis ChiA74 inhibits gram-negative bacterial and fungal pathogens of humans and plants |
| 490 0# - SERIES STATEMENT |
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| Series statement |
International Journal of Biological Macromolecules. 254, 128049, 2024, DOI: 10.1016/j.ijbiomac.2023.128049 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
The chitinase ChiA74 is synthesized by Bacillus thuringiensis and possesses a modular organization composed of four domains. In the C-terminal of the enzyme is located the chitin-binding domain (CBD), which has not been isolated as a single unit or characterized. Here, we aimed to isolate the ChiA74's CBD as a single unit, determine the binding properties, and evaluate its antimicrobial and hemolytic activities. We cloned the ChiA74's CBD and expressed it in Escherichia coli BL21. The single domain was purified, analyzed by SDS-PAGE, and characterized. The recombinant CBD (rCBD) showed a molecular mass of ?14 kDa and binds strongly to ?-chitin, with Kd and Bmax of ?4.7 ± 0.9 ?M and 1.5 ± 0.1 ?moles/g chitin, respectively. Besides, the binding potential (Bmax/Kd) was stronger for ?-chitin (?0.31) than microcrystalline cellulose (?0.19). It was also shown that the purified rCBD inhibited the growth of the clinically relevant Gram-negative bacteria (GNB) Vibrio cholerae, and V. parahemolyticus CVP2 with minimum inhibitory concentrations (MICs) of 121 ± 9.9 and 138 ± 3.2 ?g/mL, respectively, and of one of the most common GNB plant pathogens, Pseudomonas syringae with a MIC of 230 ± 13.8 ?g/mL. In addition, the rCBD possessed antifungal activity inhibiting the conidia germination of Fusarium oxysporum (MIC = 192 ± 37.5 ?g/mL) and lacked hemolytic and agglutination activities against human erythrocytes. The significance of this work lies in the fact that data provided here show for the first time that ChiA74's CBD from B. thuringiensis has antimicrobial activity, suggesting its potential use against significant pathogenic microorganisms. Future works will be focused on testing the inhibitory effect against other pathogenic microorganisms and elucidating the mechanism of action. © 2023 Elsevier B.V. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ANTIBACTERIAL ACTIVITY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ANTIFUNGAL ACTIVITY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
BACILLUS THURINGIENSIS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CHITIN-BINDING DOMAIN |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CHITINASE |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Martínez-Zavala S.A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Ortiz-Rodríguez T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Salcedo-Hernández R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Casados-Vázquez L.E. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Del Rincón-Castro M.C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Bideshi D.K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Barboza-Corona J.E. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1bYIbdHiSGJeYFIck0nTfTgD-dxTBQfin/view?usp=drivesdk">https://drive.google.com/file/d/1bYIbdHiSGJeYFIck0nTfTgD-dxTBQfin/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
