Transcriptomic and functional analyses identify genes associated with embryogenic callus formation in Neolamarckia cadamba (Record no. 55320)
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| 000 -LEADER | |
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| fixed length control field | 02723nam a2200265Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625164351.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-21250 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Transcriptomic and functional analyses identify genes associated with embryogenic callus formation in Neolamarckia cadamba |
| 490 0# - SERIES STATEMENT | |
| Series statement | Industrial Crops and Products. 216, 118686, 2024, DOI: 10.1016/j.indcrop.2024.118686 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Neolamarckia cadamba is an economically significant tree used for timber and pulp production, and as traditional medicine for various ailments. However, the absence of an effective in vitro plant regeneration system hampers its further development and promotion of superior varieties. Previous researches have highlighted the efficacy of embryogenic callus for both plant regeneration and genetic modification. To elucidate N. cadamba's embryogenesis, we conducted transcriptome profiling of leaf explants across three distinct tissue culture stages. This investigation revealed significant gene expression shifts, involving 12,963 genes. Notably, 7284 and 6652 genes displayed differential expression in ECI02 vs. ECI01 and ECI03 vs. ECI02, respectively. Functional categorization highlighted the differentially expressed transcription factors predominantly associated with hormone homeostasis and signal transduction, post-transcriptional regulation, and meristem development. Given the genes link to auxin and cytokinin signal pathways, and genes affiliate to embryogenesis identity play pivotal roles in embryogenic callus formation, we focused on these genes and found 36 of them showed differential expression patterns during embryogenic callus induction, indicating these genes potentially contribute to embryogenic callus formation. Further functional characterization confirmed that overexpression of NcBBM and NcARF5 in Nicotiana benthamiana induce callus formation without exogenous phytohormone; however, only NcBBM-induced callus exhibited shoot regeneration. These findings illuminated the molecular orchestration underlying embryogenic callus formation in N. cadamba, and genetic manipulation of these candidate genes may help to improve its tissue culture. © 2024 Elsevier B.V. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | EMBRYOGENIC CALLUS INDUCTION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | NEOLAMARCKIA CADAMBA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PLANT REGENERATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | TRANSCRIPTOME ANALYSIS |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ma Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zhao B. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zhang D. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ouyang K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Li J. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1zRcD1ReWZRAC6e7sKRN6u7u0OmKT001x/view?usp=drivesdk">https://drive.google.com/file/d/1zRcD1ReWZRAC6e7sKRN6u7u0OmKT001x/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-21250 | 25.06.2025 | 25.06.2025 | Documentos solicitados |