Isolation and fusion of protoplasts in Vanilla species (Record no. 55328)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01878nam a2200253Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625164351.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-21258 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Isolation and fusion of protoplasts in Vanilla species |
| 490 0# - SERIES STATEMENT | |
| Series statement | Current Science. 94(1), 115-120, 2008, DOI: |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Vanilla, an important flavouring material and spice, is the fermented and cured fruit of the orchid, Vanilla planifolia Andrews (syn. V. fragrans Salisb.). Origin of much of the planting material of vanilla from limited clonal source, coupled with the threat of destruction of its natural habitats, leads to a narrow genetic base being the major bottleneck in crop improvement programmes. Isolation and fusion of protoplasts was attempted to produce hybrids with desirable traits. Protoplasts were successfully isolated from V. planifolia and V. andamanica when incubated in an enzyme solution containing macerozyme R-10 (0.5%) and cellulase Onozuka R-10 (2%) for 8 h at 30°C in dark, with good yield and viability. PEG-mediated protoplast fusion between V. andamanica and V. planifolia was successful and the fusion product (heterokaryon) could easily be identified because the protoplasts of the two species differed in size and arrangement of chlorophyll. This can be helpful in gene transfer for helpful traits, especially the natural seed set and disease tolerance observed in V. andamanica. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | ISOLATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PROTOPLAST FUSION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | V. PLANIFOLIA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | VANILLA ANDAMANICA |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Divakaran M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Pillai G.S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Babu K.N. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Peter K.V. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/16NjjkTd0anmmu-QP7bzUvh5d0LBnxCsh/view?usp=drivesdk">https://drive.google.com/file/d/16NjjkTd0anmmu-QP7bzUvh5d0LBnxCsh/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-21258 | 25.06.2025 | 25.06.2025 | Documentos solicitados |