Development of an efficient protocol for doubled haploids in pearl millet using anther culture (Record no. 55380)
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| 000 -LEADER | |
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| fixed length control field | 02765nam a2200313Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625164352.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-21313 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Development of an efficient protocol for doubled haploids in pearl millet using anther culture |
| 490 0# - SERIES STATEMENT | |
| Series statement | Euphytica. 220(6), 94, 2024, DOI: https://doi.org/10.1007/s10681-024-03343-0 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Doubled haploid (DH) production technology has been a valuable tool for the development of homozygous lines through anther culture within a short period in comparison with conventional breeding methods. Doubled haploid production in pearl millet through anther culture helps to overcome inbreeding depression which occurs due to continuous selfing for homozygous line development. We developed an efficient method to develop DH plants through anther culture in pearl millet in large numbers, which is the first report in pearl millet. The method encompasses a selection of the right stage of mother plant, optimal culture media, and influence of temperature for treatment of anther for callus induction followed by plant regeneration and flow cytometric analysis for ploidy determination. Anther culture experiments were done in proprietary pearl millet genotype of Rasi seeds '1988F1' (R × R cross) for achieving better calli or embryo induction and green plant regeneration in pearl millet. A significant frequency of 35.4% calli-embryoid response in Doubled-haploid Pearl millet Media (DPM4) was achieved at incubation temperature of 32 °C for spikes pre-treatment and anther initiation. This response was further improved to 44.4% by separating anthers from spikes on fourth day of incubation. Alteration of basal salt concentration and inclusion of 12% maltose as carbon source in calli induction media resulted in a higher number of embryo-like structures (ELS) which eventually increased the regeneration up to 12.9% in Regeneration Media (RM3). These experiments resulted in a promising procedure to develop DH plants in pearl millet to minimize the timeline for pure line development and trait introgression to accelerate breeding program. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | ANDROGENESIS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | ANTHER CULTURE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | DOUBLED HAPLOID |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PEARL MILLET |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | FLOWCYTOMETRY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | REGENERATION |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Jayaraman, P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Palanisamy, D. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Shanmugam, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Marappan, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kancharla, B. R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Pathak, V. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Muthu Gounder, R. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1i5D_yYAxI4bMtXYIHy0dhQtJb1cWVi0x/view?usp=drivesdk">https://drive.google.com/file/d/1i5D_yYAxI4bMtXYIHy0dhQtJb1cWVi0x/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-21313 | 25.06.2025 | 25.06.2025 | Documentos solicitados |