Production, Purification and Immobilization of Laccase from Trametes versicolor HBB 7328 for its Role in Decolorization of Textile Dyes (Record no. 55482)
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| 000 -LEADER | |
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| fixed length control field | 02424nam a2200301Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625164354.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-21423 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Production, Purification and Immobilization of Laccase from Trametes versicolor HBB 7328 for its Role in Decolorization of Textile Dyes |
| 490 0# - SERIES STATEMENT | |
| Series statement | Indian Journal of Microbiology. 64(4), e10959, 1870-1883, 2024, DOI: 10.1007/s12088-024-01260-3 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Laccase is an extracellular enzyme that is widely used in the decolonization of textile dyes in waste water. The aim of our study was to isolate, purify, characterize and immobilize the laccase enzyme produced by Trametes versicolor HBB 7328. Purified laccase enzyme was immobilized in polyacrylamide gel to explore its ability in decolonization of textile dyes. Laccase purification process was carried out by fractionation using ammonium sulphate (80%) followed by DEAE Sepharose column (30 × 3 cm) chromatography method. Recovery and fold purification in this step were 27.35 and 16.23%. Purified laccase (named as LAC1) revealed its optimum activity at pH 5.0 and 35 °C temperature, and displayed remarkable stability in the range of 30-40 °C and in the pH range (pH 3.0-7.0). The single bands on SDS-PAGE represent the purity of LAC1 with molecular weight of 60 kDa. Both free and immobilized laccase assessed for their ability to decolorize textile dyes. Free laccase decolorized Methyl red to 72.705%, Reactive orange to 57.851%, Reactive blue to 37.231%, Bromophenol blue to 24.412% however Immobilized laccase decolorized Methyl red to 89.823%, Reactive orange to 63.151%, Reactive blue to 59.548%, Bromophenol blue to 49.421% respectively. This study proposes the role of laccase from Trametes versicolor HBB 7328 in decolonization of textile dyes. © Association of Microbiologists of India 2024. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | IMMOBILIZATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | LACCASE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | OPTIMIZATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PURIFICATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | REUSABILITY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | STABILITY |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Goyat N. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kapoor R.K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Saharan B.S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kapoor P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kumari K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Singh N. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1l_wAiJ-Xr4H8hk-4oSikZ-U2gUXVEQ3j/view?usp=drive_link">https://drive.google.com/file/d/1l_wAiJ-Xr4H8hk-4oSikZ-U2gUXVEQ3j/view?usp=drive_link</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-21423 | 25.06.2025 | 25.06.2025 | Documentos solicitados |