Production and immobilization of laccases from monoculture and co-culture of Trametes villosa and Pycnoporus sanguineus for sustainable biodegradation of ciprofloxacin (Record no. 55484)
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| fixed length control field | 02409nam a2200265Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625164354.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-21425 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Production and immobilization of laccases from monoculture and co-culture of Trametes villosa and Pycnoporus sanguineus for sustainable biodegradation of ciprofloxacin |
| 490 0# - SERIES STATEMENT | |
| Series statement | Process Biochemistry. 141, 132-143, 2024, DOI: 10.1016/j.procbio.2024.03.012 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | The presence of antibiotics in water systems and drinking water has detrimental effects on various organisms and poses health risks to both animals and humans. This study describes a sustainable approach using laccases to remove ciprofloxacin from water solutions. Laccases produced by monoculture of Trametes villosa and in co-culture with Pycnoporus sanguineus were immobilized using the method of cross-linked enzyme aggregates (CLEAs). The immobilization process was statistically optimized, and the biocatalysts were characterized and applied for biodegradation of ciprofloxacin at different concentrations. Laccase production was enhanced in co-culture conditions, yielding isoforms of molecular masses ranging from 55 to 45 kDa. Optimum conditions for immobilizing laccases by CLEAs were achieved with ammonium sulfate as a precipitant and glutaraldehyde as a cross-linker. Immobilization improved the thermal stability of the enzymes at 40 °C and 55 °C, and both forms of laccase CLEAs retained approximately 38% of their initial activity after five reuse cycles. Free and immobilized laccases demonstrated comparable efficiency in removing ciprofloxacin from aqueous solutions (at 2.5 mg L?1) with a 53-62% removal rate in the presence of 4-hydroxybenzoic acid as a natural redox mediator. The degradation of ciprofloxacin by laccases also reduced its antimicrobial activity against Escherichia coli. © 2024 Elsevier Ltd |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | ANTIBIOTICS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | BIODEGRADATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CROSS-LINKED ENZYME AGGREGATES |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | OXIDOREDUCTASE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | WHITE-ROT FUNGI |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Braga D.M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Brugnari T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Haminiuk C.W.I. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Maciel G.M. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1WK9POqWXv2G4Z8OLOVYcA1SIR48ixrDo/view?usp=drive_link">https://drive.google.com/file/d/1WK9POqWXv2G4Z8OLOVYcA1SIR48ixrDo/view?usp=drive_link</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-21425 | 25.06.2025 | 25.06.2025 | Documentos solicitados |