Exploring affinity chromatography in proteomics: A comprehensive review (Record no. 55514)

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fixed length control field 02168nam a2200301Ia 4500
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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250626080924.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-5828
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Title Exploring affinity chromatography in proteomics: A comprehensive review
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Series statement Analytica Chimica Acta, 1306, p.342513, 2024
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General note Artículo
520 3# - SUMMARY, ETC.
Summary, etc. Over the past decades, the proteomics field has undergone rapid growth. Progress in mass spectrometry and bioinformatics, together with separation methods, has brought many innovative approaches to the study of the molecular biology of the cell. The potential of affinity chromatography was recognized immediately after its first application in proteomics, and since that time, it has become one of the cornerstones of many proteomic protocols. Indeed, this chromatographic technique exploiting the specific binding between two molecules has been employed for numerous purposes, from selective removal of interfering (over)abundant proteins or enrichment of scarce biomarkers in complex biological samples to mapping the post-translational modifications and protein interactions with other proteins, nucleic acids or biologically active small molecules. This review presents a comprehensive survey of this versatile analytical tool in current proteomics. To navigate the reader, the haphazard space of affinity separations is classified according to the experiment's aims and the separated molecule's nature. Different types of available ligands and experimental strategies are discussed in further detail for each of the mentioned procedures.
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Topical term or geographic name entry element AFFINITY CHROMATOGRAPHY
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Topical term or geographic name entry element AFFINITY DEPLETION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element AFFINITY ENRICHMENT
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MASS SPECTROMETRY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PROTEIN
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Topical term or geographic name entry element PEPTIDE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PROTEOMICS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Chamrád, I.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Simerský, R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Lenobel, R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Novák, O.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/16Fz99j_6ZxA1cQS3lqOnEkq4e9Ou0fHk/view?usp=drive_link">https://drive.google.com/file/d/16Fz99j_6ZxA1cQS3lqOnEkq4e9Ou0fHk/view?usp=drive_link</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 26.06.2025   B-5828 26.06.2025 26.06.2025 Documentos solicitados