MARC details
| 000 -LEADER |
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| fixed length control field |
02138nam a2200241Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250626080925.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-9155 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Engineering all-round cellulase for bioethanol production |
| 490 0# - SERIES STATEMENT |
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| Series statement |
ACS Synthetic Biology, 12(7), p.2187-2197, 2023 |
| 500 ## - GENERAL NOTE |
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| General note |
Artículo |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
One strategy to decrease both the consumption of crude oil andenvironmental damage is through the production of bioethanol from biomass.Cellulolytic enzyme stability and enzymatic hydrolysis play important roles in thebioethanol process. However, the gradually increased ethanol concentration oftenreduces enzyme activity and leads to inactivation, thereby limiting the final ethanolyield. Herein, we employed an optimized Two-Gene Recombination Process(2GenReP) approach to evolve the exemplary cellulase CBHI for practical bioethanolfermentation. Two all-round CBHI variants (named as R2 and R4) were obtained withsimultaneously improved ethanol resistance, organic solvent inhibitor tolerance, andenzymolysis stability in simultaneous saccharification and fermentation (SSF). Notably,CBHI R4 had a 7.0- to 34.5-fold enhanced catalytic efficiency (kcat/KM) in thepresence/absence of ethanol. Employing the evolved CBHI R2 and R4 in the 1Gbioethanol process resulted in up to 10.27 percent (6.7 g/L) improved ethanol yield (ethanolconcentration) than non-cellulase, which was far more beyond than other optimization strategies. Besides bioenergy fields, thistransferable protein engineering routine holds the potential to generate all-round enzymes that meet the requirement inbiotransformation and bioenergy fields. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CELLULASE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
BIOETHANOL |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
OPTIMIZED 2GENREP |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ROBUST ENZYME |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
FERMENTATION |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Wang, M., Cui, H., Gu, C., Li, A., Qiao, J., Schwaneberg, U., ... & Huang, H. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1SHXiyHR9-H5bXayBjvfGZ1DZZR6SPHyb/view?usp=drive_link">https://drive.google.com/file/d/1SHXiyHR9-H5bXayBjvfGZ1DZZR6SPHyb/view?usp=drive_link</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
