Embryogenic callus induction, proliferation, protoplast isolation, and PEG induced fusion in Camellia oleifera (Record no. 55531)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02541nam a2200313Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250626080925.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-10438 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Embryogenic callus induction, proliferation, protoplast isolation, and PEG induced fusion in Camellia oleifera |
| 490 0# - SERIES STATEMENT | |
| Series statement | Plant Cell Tissue Organ, 157, p.75, 2024 |
| 500 ## - GENERAL NOTE | |
| General note | Artículo |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Camellia oleifera (C. oleifera) is one of the most important economic oil species in China. This study established an efficient protocol for the induction and multiplication of embryogenic callus, and the isolation, purification and Polyethylene glycol (PEG) induced fusion of protoplasts in C. oleifera. It is shown that the embryogenic callus induction was best when nodular embryogenic structures were treated with the hormone of 1.5 mg/L 2,4-Dichlorophenoxyacetic acid (2,4-D) and 1.5 mg/L 6-Benzylaminopurine (6-BA) on Murashige and Skoog (MS) medium, and the highest induction rate of embryogenic callus was 18.82% after 25 days of culture. Embryogenic callus was further multiplied on woody plant medium (WPM) with 0.75 mg/L 2,4-D, pH 5.8. The Proliferating rate of embryogenic callus reached 1.8 after 20 days of culture. The maximum yield (3.16 × 106 protoplasts per gram fresh tissue) and viability (93%) of embryogenic callus protoplast were reached when embryogenic callus was treated in dark for 24 h, vacuumed at -0.03 MPa for 10 min, digested of embryogenic callus with 0.75% (w/v) Cellulase R-10 and 0.5% (w/v) Macerozyme R-10 for 6 hours at 28ºC. The purification effect was the best when using W buffer as a cleaning agent centrifuged 400 revolutions per minute (rpm) for 4 min by precipitation method. Moreover, the maximum fusion efficiency (11.18%) of mesophyll protoplasts and embryogenic callus protoplasts was obtained with 30% PEG 6000 for 15 min. The study lays a foundation for future research in somatic hybridization in C. oleifera. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CAMELLIA OLEÍFERA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | EMBRYOGENIC CALLUS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PROTOPLAST ISOLATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PURIFICATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | FUSION |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | He, X.-Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Xu, L.-J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Xu, X.-S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Yi, D.-D. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hou, S.-L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Yuan, D.-Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Xiao, S.-X. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1dzPSvf0H7dhCgkQjeXO3mE2odbFndJOs/view?usp=drive_link">https://drive.google.com/file/d/1dzPSvf0H7dhCgkQjeXO3mE2odbFndJOs/view?usp=drive_link</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 26.06.2025 | B-10438 | 26.06.2025 | 26.06.2025 | Documentos solicitados |