The Inoue method for preparation and transformation of competent Escherichia coli:"Ultracompetent" cells (Record no. 55548)

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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250626080925.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-15174
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Title The Inoue method for preparation and transformation of competent Escherichia coli:"Ultracompetent" cells
490 0# - SERIES STATEMENT
Series statement Cold Spring Harbor Protocol, 6, p.pdb-prot101196, 2020
500 ## - GENERAL NOTE
General note Artículo
520 3# - SUMMARY, ETC.
Summary, etc. This protocol differs from other transformation procedures in that the bacterial culture is grown at 18 C rather than the conventional 37 C. Otherwise, the protocol is unremarkable and follows a fairly standard course. Under standard laboratory conditions, efficiencies of 1× 10 8 to 3× 10 8 transformed colonies/µg of plasmid DNA are typical.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TRANSFORMATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element COMPETENT ESCHERICHIA COLI
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Green, M. R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Sambrook, J.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1iANnzpPpF1gkFq9epIYnTLA1XNtna6zw/view?usp=drive_link">https://drive.google.com/file/d/1iANnzpPpF1gkFq9epIYnTLA1XNtna6zw/view?usp=drive_link</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 26.06.2025   B-15174 26.06.2025 26.06.2025 Documentos solicitados