Cryopreservation of zygotic embryos derived from BBTV resistant accessions of wild banana Musa acuminata (Record no. 55586)

MARC details
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fixed length control field 02869nam a2200361Ia 4500
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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250626080926.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-20378
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245 10 - TITLE STATEMENT
Title Cryopreservation of zygotic embryos derived from BBTV resistant accessions of wild banana Musa acuminata
490 0# - SERIES STATEMENT
Series statement South African Journal of Botany, 173, p.255-265, 2024
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General note Artículo
520 3# - SUMMARY, ETC.
Summary, etc. The wild banana species Musa acuminata encompasses numerous subspecies, some of which serve as ancestors of edible bananas. Preserving these subspecies is critical for food security, especially in regions where bananas are staple crops. While cryopreservation of M. acuminata subsp. burmanicoides zygotic embryos using air dehydration have shown high success rates, this method has not been effective for other subspecies. With at least 15 subspecies displaying various morphological traits, including seed morphology, alternative cryopreservation methods warrant exploration. In a recent study, successful cryopreservation using the droplet-vitrification method was achieved for mature zygotic embryos of accession of M. acuminata subspecies tomentosa, rutilifes, and malaccensis, all of which are resistant to the Banana bunchy top virus (BBTV). Using this approach, subjecting embryos to dehydration for 20 min in PVS2 solution and employing a regeneration medium comprising MS medium supplemented with benzyladenine and thidiazuron resulted in a success rate of 77.4 % (86 % survival rate × 90 % regrowth rate) for subsp. tomentosa and 50.4 % (70 % survival rate × 72 % regrowth rate) for subsp. malaccensis. Conversely, for subsp. rutilifes, dehydration for 10 min led to a success rate of 15.6 % (30 % survival rate × 52 % regrowth rate). Subspecies emerged as the primary factor influencing embryo regrowth, suggesting the need for protocol optimizations tailored to other subspecies. These findings highlight the potential of droplet-vitrification as a long-term preservation method for certain subspecies, while underscoring the necessity for further optimization for the other subspecies of M. acuminata.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MUSA ACUMINATA SUBSP. MALACCENSIS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SUBSP. RUTILIFES
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SUBSP. TOMENTOSA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CONSERVATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element VITRIFICATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DROPLET-VITRIFICATION
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Roostika, I.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Rahayu, E.M.D.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Prawestri, A.D.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Poerba, Y.S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Handayani, T.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Ahmad, F.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Widjaya, A.H.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Martanti, D.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Herlina
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Latifah, D.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1k0HHDYh2qD5uTb8_e4onSlZLpfH3qo6m/view?usp=drive_link">https://drive.google.com/file/d/1k0HHDYh2qD5uTb8_e4onSlZLpfH3qo6m/view?usp=drive_link</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 26.06.2025   B-20378 26.06.2025 26.06.2025 Documentos solicitados