MARC details
| 000 -LEADER |
| fixed length control field |
02869nam a2200361Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
| control field |
20250626080926.0 |
| 040 ## - CATALOGING SOURCE |
| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-20378 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
| Title |
Cryopreservation of zygotic embryos derived from BBTV resistant accessions of wild banana Musa acuminata |
| 490 0# - SERIES STATEMENT |
| Series statement |
South African Journal of Botany, 173, p.255-265, 2024 |
| 500 ## - GENERAL NOTE |
| General note |
Artículo |
| 520 3# - SUMMARY, ETC. |
| Summary, etc. |
The wild banana species Musa acuminata encompasses numerous subspecies, some of which serve as ancestors of edible bananas. Preserving these subspecies is critical for food security, especially in regions where bananas are staple crops. While cryopreservation of M. acuminata subsp. burmanicoides zygotic embryos using air dehydration have shown high success rates, this method has not been effective for other subspecies. With at least 15 subspecies displaying various morphological traits, including seed morphology, alternative cryopreservation methods warrant exploration. In a recent study, successful cryopreservation using the droplet-vitrification method was achieved for mature zygotic embryos of accession of M. acuminata subspecies tomentosa, rutilifes, and malaccensis, all of which are resistant to the Banana bunchy top virus (BBTV). Using this approach, subjecting embryos to dehydration for 20 min in PVS2 solution and employing a regeneration medium comprising MS medium supplemented with benzyladenine and thidiazuron resulted in a success rate of 77.4 % (86 % survival rate × 90 % regrowth rate) for subsp. tomentosa and 50.4 % (70 % survival rate × 72 % regrowth rate) for subsp. malaccensis. Conversely, for subsp. rutilifes, dehydration for 10 min led to a success rate of 15.6 % (30 % survival rate × 52 % regrowth rate). Subspecies emerged as the primary factor influencing embryo regrowth, suggesting the need for protocol optimizations tailored to other subspecies. These findings highlight the potential of droplet-vitrification as a long-term preservation method for certain subspecies, while underscoring the necessity for further optimization for the other subspecies of M. acuminata. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
| Topical term or geographic name entry element |
MUSA ACUMINATA SUBSP. MALACCENSIS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
| Topical term or geographic name entry element |
SUBSP. RUTILIFES |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
| Topical term or geographic name entry element |
SUBSP. TOMENTOSA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
| Topical term or geographic name entry element |
CONSERVATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
| Topical term or geographic name entry element |
VITRIFICATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
| Topical term or geographic name entry element |
DROPLET-VITRIFICATION |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
| Personal name |
Roostika, I. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
| Personal name |
Rahayu, E.M.D. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
| Personal name |
Prawestri, A.D. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
| Personal name |
Poerba, Y.S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
| Personal name |
Handayani, T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
| Personal name |
Ahmad, F. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
| Personal name |
Widjaya, A.H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
| Personal name |
Martanti, D. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
| Personal name |
Herlina |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
| Personal name |
Latifah, D. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1k0HHDYh2qD5uTb8_e4onSlZLpfH3qo6m/view?usp=drive_link">https://drive.google.com/file/d/1k0HHDYh2qD5uTb8_e4onSlZLpfH3qo6m/view?usp=drive_link</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |