The dynamics of sugars, hormones, and cell wall epitopes dictate early somatic embryogenesis in passion fruit (Passiflora edulis Sims) (Record no. 55633)

MARC details
000 -LEADER
fixed length control field 02941nam a2200325Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250626083335.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-21564
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245 10 - TITLE STATEMENT
Title The dynamics of sugars, hormones, and cell wall epitopes dictate early somatic embryogenesis in passion fruit (Passiflora edulis Sims)
490 0# - SERIES STATEMENT
Series statement Plant Cell, Tissue and Organ Culture, 157(1), p.20, 2024
500 ## - GENERAL NOTE
General note Artículo
520 3# - SUMMARY, ETC.
Summary, etc. Sugars, hormones and plant cell wall components regulate many aspects of somatic embryogenesis which requires detailed investigations of the process. In the present study, we investigated the histology, chemical composition of the cell wall, sugar and nitrogen metabolism, and hormonal profile associated with the induction and formation of embryogenic calluses in Passiflora edulis Sims. Zygotic embryos were cultured in induction medium supplemented with 72.4 µM dichlorophenoxyacetic acid and 4.4 µM 6-benzyladenine for 30 days. The zygotic embryo consisted of a uniseriate protoderm and an undifferentiated mesophyll. After 20 days, globular-stage somatic embryos were observed at the periphery of embryogenic calluses. During callus formation, the cell wall of dividing cells showed high levels of methyl-esterified homogalacturonan (HGs) epitopes, recognized for JIM7 antibody. Epitopes of heteromannans (LM21) were identified only in the initial explants, whereas epitopes of xyloglucans (LM15) appeared throughout the morphogenetic process. Cells undergoing transdifferentiation in embryogenic calluses exhibited a drop in epitopes recognized for JIM7 and LM15 and absence of JIM5 labeling. Lipids and proteins were metabolized during early somatic embryogenesis. In contrast, hexose, starch, and amino acid concentrations increased. Hormonal dynamics were also altered: while indole-3-acetic acid and cytokinins decreased during the embryogenic process, jasmonic acid and ethylene showed the opposite trend, indicating a possible role of these stress hormones in the early somatic embryogenesis of passion fruit. These results shed light on the structural and physiological aspects of passion fruit somatic embryogenesis which may help optimize regeneration via somatic embryogenesis of this important commercial passion fruit species.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element BIOCHEMICAL PROFILING
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CARBOHYDRATE METABOLISM
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element EMBRYOGENIC CALLUS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element EMBRYOGENIC MARKERS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element IMMUNOHISTOCHEMISTRY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PASSIFLORACEAE
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Dias, L. L. L.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Silva, L. A. S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Vieira, L. M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Machado, M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Nunes-Nesi, A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kuster, V. C.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Rocha, D. I.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1xA8IQRVKGukSJrOaS5vML_XbSsT0I2Pa/view?usp=drive_link">https://drive.google.com/file/d/1xA8IQRVKGukSJrOaS5vML_XbSsT0I2Pa/view?usp=drive_link</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 26.06.2025   B-21564 26.06.2025 26.06.2025 Documentos solicitados