Organization and expression of two Arabidopsis DREB2 genes encoding DRE-binding proteins involved in dehydration- and high-salinity-responsive gene expression (Record no. 55683)

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control field MX-MdCICY
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control field 20250626083336.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-21615
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Title Organization and expression of two Arabidopsis DREB2 genes encoding DRE-binding proteins involved in dehydration- and high-salinity-responsive gene expression
490 0# - SERIES STATEMENT
Series statement Plant Molecular Biology, 42, p.657-665, 2000
500 ## - GENERAL NOTE
General note Artículo
520 3# - SUMMARY, ETC.
Summary, etc. In plants, a cis-acting element, DRE/CRT, is involved in ABA-independent gene expression in response to dehydration and low-temperature stress. To understand signal transduction pathways from perception of the dehydration stress signal to gene expression, we characterized a gene family for DRE/CRT-binding proteins DREB2A and DREB2B in Arabidopsis thaliana. Northern analysis showed that both genes are induced by dehydration and high-salt stress. Organ-specific northern analysis with gene-specific probes showed that these genes are strongly induced in roots by high-salt stress and in stems and roots by dehydration stress. The DREB2A gene is located on chromosome 5, and DREB2B on chromosome 3. We screened an Arabidopsis genomic DNA library with cDNA fragments of DREB2A and DREB2B as probes, and isolated DNA fragments that contained 50 -flanking regions of these genes. Sequence analysis showed that both genes are interrupted by a single intron at identical positions in their leader sequence. Several conserved sequences were found in the promoter regions of both genes. The β-glucuronidase (GUS) reporter gene driven by the DREB2 promoters was induced by dehydration and high-salt stress in transgenic Arabidopsis plants.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DREB
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DRE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ARABIDOPSIS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DEHYDRATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element HIGH SALT STRESS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PROMOTER
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Nakashima, K.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Shinwari, Z. K.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Sakuma, Y.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Seki, M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Miura, S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Shinozaki, K.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Yamaguchi-Shinozaki, K. 
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/151oz1IWxRimnAsRdmORutzkczqxfvYk-/view?usp=drive_link">https://drive.google.com/file/d/151oz1IWxRimnAsRdmORutzkczqxfvYk-/view?usp=drive_link</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 26.06.2025   B-21615 26.06.2025 26.06.2025 Documentos solicitados