Influence of Nitrogen Balance of Culture Medium on Norway Spruce [Picea abies (L.) Karst] Somatic Polyembryogenesis: High Frequency Establishment of Embryonal-Suspensor Mass Lines from Mature Zygotic Embryos (Record no. 55690)

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control field 20250626083336.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-21623
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245 10 - TITLE STATEMENT
Title Influence of Nitrogen Balance of Culture Medium on Norway Spruce [Picea abies (L.) Karst] Somatic Polyembryogenesis: High Frequency Establishment of Embryonal-Suspensor Mass Lines from Mature Zygotic Embryos
490 0# - SERIES STATEMENT
Series statement Journal of Plant Physiology 142 (6):735-741, 1993
500 ## - GENERAL NOTE
General note Artículo
520 3# - SUMMARY, ETC.
Summary, etc. Proliferating embryonal-suspensor masses (ESMs) were initiated from mature open-pollinated seeds of a northern Norway spruce (Picea abies (L.) Karst) family possessed a relatively low level of embryogenic activity. Different nitrogen content modifications of the half-strength von Arnold and Eriksson (~ LP) basal medium were tested for both the induction of ESM formation and the establishment of ESM lines. Both organic and inorganic nitrogen strongly affected the ESM line establishment. Amide nitrogen (asparagine or glutamine) was an obligatory prerequisite for the ESM line establishment and prolonged maintenance. The optimal ammonium! nitrate molar ratio in the presence of 3.0 mM glutamine was about 0.2. The highest frequency ESM line establishment (33 percent) was obtained with 7.5 mM ammonium, 33.8 mM nitrate and 3.0 mM glutamine. Somatic embryo maturation was determined by the combined effects of the nitrogen balances of maintenance and maturation media. After a two-step maturation procedure, mature somatic embryos were successfully germinated and transferred ex vitrum.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PICEA ABIES
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ESTABLISHMENT OF EMBRYONAL-SUSPENSOR MASS LINES
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element NITROGEN SOURCES
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element NORWAY SPRUCE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SOMATIC POLYEMBRYOGENESIS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Bozhkov, P. V.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Mikhlina, S. B.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Shiryaeva, G. A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Lebedenko, L. A.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/13f0gQ5e7H50JooorsW-9AEpelw0BTNwe/view?usp=drive_link">https://drive.google.com/file/d/13f0gQ5e7H50JooorsW-9AEpelw0BTNwe/view?usp=drive_link</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 26.06.2025   B-21623 26.06.2025 26.06.2025 Documentos solicitados