Altering Endogenous Cytokinin Content by GmCKX13 as a Strategy to Develop DroughtTolerant Plants (Record no. 55759)
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| 000 -LEADER | |
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| fixed length control field | 02809nam a2200313Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250626090915.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-21694 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Altering Endogenous Cytokinin Content by GmCKX13 as a Strategy to Develop DroughtTolerant Plants |
| 490 0# - SERIES STATEMENT | |
| Series statement | Plant Stress, p.100678, 2024 |
| 500 ## - GENERAL NOTE | |
| General note | Artículo |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Climate-resilient crops are essential to meet the growing food demands of an ever-increasing world population. The phytohormone cytokinins (CKs) have been well established to regulate plant adaptation to drought. Previously, we reported that GmCKX13, encoding a CK oxidase/dehydrogenase in soybean (Glycince max), was differentially expressed under drought. Here, we further characterized its in planta function to assess if GmCKX13 could be a candidate gene to develop drought-tolerant crops. Transgenic Arabidopsis (35S:GmCKX13) plants ectopically and constitutively expressing GmCKX13 using the 35S promoter had an increase in root length, while showing a reduction in shoot height and biomass. CK contents were reduced in 35S:GmCKX13 plants, coupled with the reduced expression of all AtCKX genes and increased expression of all Arabidopsis isopentenyl transferases (AtIPTs), except AtIPT2 and AtIPT9 that are responsible for the production of cis-zeatin-type CKs. The 35S:GmCKX13 plants showed improved tolerance to drought and more sensitivity to the treatment with exogenous abscisic acid (ABA). Under the control of the drought-responsive promoter RD29A, the transgenic RD29A:GmCKX13 Arabidopsis plants had a similar phenotype as that of the wild-type (WT) plants under normal conditions. Under dehydration, we found a significant increase in the expression of the transgene coupled with a higher leaf relative water content in RD29A:GmCKX13 plants when compared with WT plants. In consistence with this finding, the RD29A:GmCKX13 plants exhibited higher survival rates and 30 percent higher seed yield than the WT plants under drought conditions. Taken together, our results demonstrated that GmCKX13 is an excellent gene for developing drought-tolerant crops by altering endogenous CK levels and ABA responsiveness when being driven by a drought-responsive promoter. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CYTOKININ MODULATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | DROUGHT TOLERANCE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | GMCKX13 |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | RD29A |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | TRANSGENIC |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Le, D. T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Van Ha, C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Nguyen, K. H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Chu, H. D. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zhu, C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Li, W. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Tran, L. S. P. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/13FIdaytlt5nvOgsX8hfS5CqgLx3nLUDS/view?usp=drive_link">https://drive.google.com/file/d/13FIdaytlt5nvOgsX8hfS5CqgLx3nLUDS/view?usp=drive_link</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 26.06.2025 | B-21694 | 26.06.2025 | 26.06.2025 | Documentos solicitados |