Cyclic nucleotide-gated ion channel 20 regulates melatonin-induced calcium signaling and cold tolerance in watermelon (Record no. 55824)
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| fixed length control field | 02669nam a2200253Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250626090917.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-21762 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Cyclic nucleotide-gated ion channel 20 regulates melatonin-induced calcium signaling and cold tolerance in watermelon |
| 490 0# - SERIES STATEMENT | |
| Series statement | Plant Physiology, kiae630, https://doi.org/10.1093/plphys/kiae630, 2024 |
| 500 ## - GENERAL NOTE | |
| General note | Artículo |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Melatonin plays a crucial role in regulating plant cold tolerance, but the mechanisms underlying signal transduction remain elusive. In this study, we discovered that overexpression of the melatonin biosynthetic gene caffeic acid O-methyltransferase1 (COMT1) enhanced watermelon (Citrullus lanatus) cold tolerance, accompanied by the accumulation of cytosolic free calcium ([Ca2+]cyt), a stimulation of Ca2+ influx, and upregulation of four Ca2+-permeable channel genes (CNGC2/10/17/20). Conversely, knockout of COMT1 exhibited contrasting effects compared to its overexpression. Knocking out the four CNGC genes revealed that only CNGC20 mediates melatonin-induced Ca2+ influx in response to cold stimuli. CNGC20 deletion impeded watermelon callus redifferentiation, prompting us to employ a virus-induced gene silencing strategy to suppress its expression. Silencing CNGC20 compromised COMT1 overexpression-induced [Ca2+]cyt accumulation, Ca2+ influx, and watermelon cold tolerance. Yeast two-hybrid, bimolecular fluorescence complementation, firefly luciferase complementation imaging, and pull-down assays revealed an interaction between CNGC20 and calmodulin7 (CaM7). Overexpressing CaM7 inhibited melatonin-induced [Ca2+]cyt accumulation, Ca2+ influx, and watermelon cold tolerance. Conversely, silencing CaM7 increased [Ca2+]cyt accumulation, Ca2+ influx, and cold tolerance, whereas COMT1 overexpression failed to further enhance these responses in CaM7-silenced plants, indicating the negative regulation role of CaM7 in melatonin-mediated cold responses. Overall, these findings provide insights into the molecular mechanisms underlying melatonin-enhanced plant cold tolerance via Ca2+ signaling, holding potential for breeding/engineering cold-tolerant cucurbit varieties. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Chang, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Guo, Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Li, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Liu, L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Liu, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Yuan, L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Jalal Ahammed, G. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1JevKPlMOH9rNdiacynKFOeZ01AOkze6f/view?usp=drive_link">https://drive.google.com/file/d/1JevKPlMOH9rNdiacynKFOeZ01AOkze6f/view?usp=drive_link</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 26.06.2025 | B-21762 | 26.06.2025 | 26.06.2025 | Documentos solicitados |