MARC details
| 000 -LEADER |
|---|
| fixed length control field |
03882nam a22004575i 4500 |
| 001 - CONTROL NUMBER |
|---|
| control field |
978-1-4020-6040-3 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
DE-He213 |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20251006084528.0 |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION |
|---|
| fixed length control field |
cr nn 008mamaa |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
100301s2007 ne | s |||| 0|eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
|---|
| International Standard Book Number |
9781402060403 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
|---|
| International Standard Book Number |
99781402060403 |
| 024 7# - OTHER STANDARD IDENTIFIER |
|---|
| Standard number or code |
10.1007/978-1-4020-6040-3 |
| Source of number or code |
doi |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER |
|---|
| Classification number |
614.5999 |
| Edition information |
23 |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Buzdin, Anton A. |
| Relator term |
editor. |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Nucleic Acids Hybridization Modern Applications |
| Medium |
[electronic resource] / |
| Statement of responsibility, etc. |
edited by Anton A. Buzdin, Sergey A. Lukyanov. |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
|---|
| Place of production, publication, distribution, manufacture |
Dordrecht : |
| Name of producer, publisher, distributor, manufacturer |
Springer Netherlands, |
| Date of production, publication, distribution, manufacture, or copyright notice |
2007. |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
XIX, 314 p. |
| Other physical details |
online resource. |
| 336 ## - CONTENT TYPE |
|---|
| Content type term |
text |
| Content type code |
txt |
| Source |
rdacontent |
| 337 ## - MEDIA TYPE |
|---|
| Media type term |
computer |
| Media type code |
c |
| Source |
rdamedia |
| 338 ## - CARRIER TYPE |
|---|
| Carrier type term |
online resource |
| Carrier type code |
cr |
| Source |
rdacarrier |
| 347 ## - DIGITAL FILE CHARACTERISTICS |
|---|
| File type |
text file |
| Encoding format |
PDF |
| Source |
rda |
| 505 0# - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
Nucleic Acids Hybridization: Potentials and Limitations -- Selective Suppression of Polymerase Chain Reaction and Its Most Popular Applications -- Suppression Subtractive Hybridization -- Stem-Loop Oligonucleotides as Hybridization Probes and Their Practical Use in Molecular Biology and Biomedicine -- Normalization of cDNA Libraries -- Primer Extension Enrichment Reaction (PEER) and Other Methods for Difference Screening -- Subtractive Hybridization with Covalently Modified Oligonucleotides -- Coincidence Cloning: Robust Technique for Isolation of Common Sequences -- DNA Hybridization in Solution for Mutation Detection -- Current Attempts to Improve the Specificity of Nucleic Acids Hybridization -- Concepts on Microarray Design for Genome and Transcriptome Analyses. |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
Watson-Crick hybridization of complementary sequences in nucleic acids is one of the most important processes necessary for molecular recognition in vivo, as well as nucleic acid identification and isolation. This book is devoted to a large family of in vitro DNA hybridization-based experimental techniques. A wide spectrum of experimental tasks covered by these approaches includes finding differential sequences in both genomic DNAs and mRNAs, genome walking, multiplex PCR, cDNA library construction starting from minute amount of total RNA, rapid amplification of cDNA 5' and 3' ends, effective smoothing of the concentrations of rare and abundant transcripts in cDNA libraries, recovery of promoter active repeats and differentially methylated genomic DNA, identification of common sequences in genomic or cDNA sources, new gene mapping, finding evolutionary conserved DNA and both single-nucleotide and extended mutation discovery or large-scale monitoring. Several approaches, such as microarray hybridization, have become extremely popular tools for specialists in biochemistry and in biomedicine, whereas the potential of many other advantageous techniques seems to be underestimated now. The international team of the authors of this book has tried both to elucidate the current state-of-art in hybridization techniques and to help the readers in choosing an appropriate method for performing an experiment in the most efficient way. Enclosed experimental protocols along with both comprehensive and detailed method descriptions make this truly universal book useful to all those interested in the modern life science methodologies. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
MEDICINE. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
ONCOLOGY. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
HUMAN GENETICS. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
MEDICAL VIROLOGY. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
BIOMEDICINE. |
| 650 24 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
CANCER RESEARCH. |
| 650 24 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
HUMAN GENETICS. |
| 650 24 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
VIROLOGY. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Lukyanov, Sergey A. |
| Relator term |
editor. |
| 710 2# - ADDED ENTRY--CORPORATE NAME |
|---|
| Corporate name or jurisdiction name as entry element |
SpringerLink (Online service) |
| 773 0# - HOST ITEM ENTRY |
|---|
| Title |
Springer eBooks |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY |
|---|
| Relationship information |
Printed edition: |
| International Standard Book Number |
9781402060397 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
|---|
| Uniform Resource Identifier |
<a href="http://dx.doi.org/10.1007/978-1-4020-6040-3">http://dx.doi.org/10.1007/978-1-4020-6040-3</a> |
| Public note |
Ver el texto completo en las instalaciones del CICY |
| 912 ## - |
|---|
| -- |
ZDB-2-SBL |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Libros electrónicos |
