Construction of an infectious clone of citrus chlorotic dwarf-associated virus and confirmation of its pathogenicity. (Record no. 61897)

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fixed length control field 01990nam a2200253Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20251009160706.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-21806
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 251009s9999 xx 000 0 und d
245 10 - TITLE STATEMENT
Title Construction of an infectious clone of citrus chlorotic dwarf-associated virus and confirmation of its pathogenicity.
490 0# - SERIES STATEMENT
Series statement Plant Disease, 108(11), p.3393-3399, 2024
500 ## - GENERAL NOTE
General note Artículo
520 3# - SUMMARY, ETC.
Summary, etc. Citrus chlorotic dwarf disease (CCDD) seriously affects the citrus industry. Citrus chlorotic dwarf-associated virus (CCDaV) is speculated to be the causal agent ofCCDD. Howcvcr, this spcculation has not been confirrncd by fulfilling Koch's postulatcs. In this study, an infcctio us clone comprising a 1.6-fold tandem CCDaV genome in the binary vector pBinPLUS was eonstructed and agro-inoculated into 'Eurcka' lemon (Citrus liman) scedlings through vacuum infiltration. At 60 days postinoculation, 25 percent of the 'Eurcka' lcmon seedlings dcvelopcd symptoms of crinkling and curling that wcre the same as those associated with the wild-type virus. Western blotting and graft transmission assays confirrned that the infectious clone systcmically infected 'Eureka' lemon seedlings. In addition, CCDaV can establish infection on thrcc more Citrus species and one hybrid, although at diffcrent infection rates. These findings support that CCDaV is the primary causal agent ofCCDD. The infectious CCDaV clone will allow furthcr studies on the functions ofviral proteins and molecular interactions ofCCDaV with its hosts.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CAUSATIVE AGENT
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DETECTION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ETIOLOGY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element HOST RANGC
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PATHOGENICITY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element VIRUSES AND VIROIDS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Zhao, J.;Zhang, S.;Wang, J.;Lou, B.;Zhou, Y.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1RUKuo-KJWIRrD_zskqnsy6DgjS3Zdipv/view?usp=drive_link">https://drive.google.com/file/d/1RUKuo-KJWIRrD_zskqnsy6DgjS3Zdipv/view?usp=drive_link</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 09.10.2025   B-21806 09.10.2025 09.10.2025 Documentos solicitados